hsa-miR-34b-3p与CDK4在骨肉瘤组织中的表达水平及临床意义

Expressions of hsa-miR-34b-3p and CDK4 in Osteosarcoma and Their Clinical Significance

目的探究hsa-miR-34b-3p(miR-34b)与CDK4在骨肉瘤中表达水平,并分析临床意义。方法选取100例冻存骨肉瘤组织及其对应的癌旁组织标本。使用qRT-PCR检测组织中miR-34b表达水平。使用WB检测CDK4蛋白的表达水平。结果与癌旁非肿瘤组织相比,骨肉瘤组织中miR-34b表达水平显著降低,而CDK4蛋白的表达显著增高,差异具有统计学意义(P<0.05)。在骨肉瘤组织中,miR-34b的表达随肿瘤分期进展而逐渐降低,而CDK4蛋白的表达逐渐增高,miR-34b表达与CDK4蛋白表达呈负相关。在软组织浸润和转移者中miR-34b的表达水平显著低于软组织无浸润和无转移者,而CDK4蛋白表达则相反。结论在骨肉瘤组织中,miR-34b的表达随临床分期进展而逐渐降低,可能通过负向调控CDK4表达而在骨肉瘤发生发展过程中发挥作用。

Objective To investigate the expression of hsa-miR-34b-3p（miR-34b） and CDK4 in osteosarcoma,and analyze the clinical significance. Methods 100 cases of cryosurgery osteosarcoma tissues and their corresponding adjacent tissue specimens were selected.qRT-PCR was used to detect miR-34b expression in the tissues.WB was used to detect the expression level of CDK4 protein. Results Compared with adjacent tissue,the expression of miR-34b in osteosarcoma tissue significantly reduced while the expression of CDK4 protein significantly increased（ P 〈0.05）.In osteosarcoma tissues,the expression of miR-34b gradually decreased as the stage of tumor progressed,while the expression of CDK4 protein gradually increased,and the expression of miR-34b was negatively correlated with CDK4 protein.The expression of miR-34b in soft tissue infiltrating and metastatic patients was significantly lower than those in soft tissue without invasion and metastasis.However,CDK4 protein expression was the opposite. Conclusion In osteosarcoma tissues,the expression of miR-34b decreases gradually with the progression of clinical stage,and may play a role in the development of osteosarcoma by negatively regulating the expression of CDK4.