丛枝菌根真菌与蚯蚓对马缨丹镉吸收和抗氧化酶活性的影响

Effect of Arbuscular Mycorrhizal Fungi and Earthworms on Cadmium Uptake and Antioxidant Enzyme Activities of Lantana camara in Cd-Polluted Soils

采用盆栽试验,研究不同施镉水平(0.25、7.15、28.37 mg/kg)土壤中单独或联合接种丛枝菌根真菌(Arbuscular Mycorrhizal Fungi,AMF)和蚯蚓对马缨丹(Lantana camara)的生物量、镉吸收和抗氧化酶活性的影响.结果表明,镉抑制了马缨丹的生长,降低了地上部磷的含量和过氧化氢酶(CAT)、超氧化物歧化酶(SOD)的活性,提高了过氧化物酶(POD)活性和丙二醛(MDA)含量;AMF能较好地侵染马缨丹根系,接种AMF提高了马缨丹地上部的干质量、磷含量、镉含量以及SOD活性,降低了CAT活性和MDA含量;添加蚯蚓增加了马缨丹地上部镉含量,降低了CAT、POD和SOD活性,提高了MDA含量.多因素方差分析表明:接种AMF和蚯蚓的主效应比它们的交互作用对马缨丹镉吸收和抗氧化酶活性的影响更重要.相关性分析显示抗氧化酶活性与地上部镉和磷含量显著相关,这表明接种AMF和蚯蚓可能是通过对马缨丹吸收镉和磷的作用来影响抗氧化酶活性.

A pot experiment was conducted to investigate the effects of earthworm and arbuscular mycorrhiza fungi（ AMF） respectively and their combined inoculation on the biomass,Cd accumulation and antioxidant enzyme activity of Lantana camara in different Cd-spiked soils（ 0.25,7.15 and 28.37 mg/kg soil）. Results showed that Cd inhibited plant growth decreased the concentration of P and the activity of catalase（ CAT） and superoxide dismutase（ SOD）,and increased the activity of peroxidase（ POD） and the content of malonaldehyde（ MDA） in the shoots of L. camara. AMF associated with L. camara well,and AMF inoculation increased dry weight,P and Cd concentration,SOD activity but decreased CAT activity and MDA content in the shoots. Earthworm addition increased Cd concentration and MDA content and decreased the activity of CAT,POD and SOD in the shoots. The full factorial analysis of variance indicated that the main effects of AMF and earthworm on the Cd-uptake and antioxidant activity of L. camara were more important than their interactive effects. Correlation analysis showed significant correlations between the activity of all antioxidant enzymes and P and Cd concentration in the shoots,indicating that earthworm and AMF inoculation may influence the activity of antioxidant enzymes through the effects of P and Cd absorption in the shoots of L. camara.