TSA对不同抗旱性花生品种响应干旱过程中光合特性及相关基因表达的影响

Effect of TSA on Photosynthesis-Related Gene Expression in Different Drought-Resistant Peanut Cultivars Subjected to Drought Stress

以2种不同抗旱性(抗旱品种粤油7号和敏旱品种汕优523)花生为材料,研究组蛋白去乙酰化酶抑制剂曲古抑菌素A(Trichostatin A,TSA)在模拟干旱条件下对花生幼苗叶片叶绿素质量分数(w)、w(叶绿素a)∶w(叶绿素b)、荧光特性和光合相关基因表达的影响.结果表明:粤油7号的总电子传递速率(ETR)和PSⅡ光量子产率(ΦPSⅡ)对干旱的响应较汕优523强,1μmol/L TSA处理12 h能使2个品种花生在干旱条件下保持较高的光合效率;经TSA处理的花生在干旱胁迫下,叶片Ah LHCB3和Ah PORA表达上调,AhRUBP表达下调,差异均有统计学意义(P<0.05),2种不同抗旱性品种花生在TSA影响下对干旱的响应趋势一致,响应幅度和时间有差异.

The changes of chlorophyll content, fluorescence characteristics and expression of photosynthesis-related genes in two peanut cultivars （Yueyou 7 and Shanyou 523） subjected to simulated drought stress were investigated by the treatment of trichostatin A （TSA）, a histone deacetylase inhibitor. The results show that the response of Yueyou 7 to drought stress on total electron transfer rate （ETR） and PS Ⅱ quantum yield （ Φ PSⅡ ） is stronger than that of Shanyou 523. High photosynthetic efficiency of both cultivars of peanut can be maintained under drought stress with the treatment of 1 μmol/L TSA for 12 h. Meanwhile, with the treatment above, the expression level of AhLHCB 3 and AhPORA increase significantly（ P 〈0.05）, while that of AhRuBP decreases（ P 〈0.05）. The response trend of both cultivars to drought stress under the effect of TSA is similar but different in response level and time.