丁虫腈乳油的高效液相色谱分析方法研究

HPLC Analysis Research on Flufiprole EC

采用高效液相色谱法,以甲醇+乙腈+水(用磷酸调pH=3.0)溶液为流动相,使用ZORBAX SB-C185μm为填料的不锈钢柱,检测波长为220 nm,对丁虫腈乳油进行定量分析。结果表明,该方法的标准偏差为0.14,相对标准偏差为0.91%,平均回收率为99.17%,丁虫腈的检测浓度在0.01~0.50 mg/m L范围内的线性相关系数为0.999 7。

To establish a HPLC method for determination of flufiprole EC, methanol, acetonitrile and water（ p H was adjusted to 3. 0 with phosphoric acid）were used as mobile phase,with a ZORBAX SB-C185 μm as chromatographic column,and UV detection wavelength was set at 220 nm.The standard deviation of this method was 0.14, the variation coefficient was 0.91%,and the average recovery rate was 99.17%.The standard curve was linear over the range of 0.01-0.50 mg/m L with linear correlation coefficient as 0.999 7.