基于转录组测序的紫色秋葵花青素合成相关基因研究

Utilizing Transcriptome of Purple Abelmoschus esculentus for Sequence Analysis on Anthocyanidin Gene

紫色秋葵富含花青素,因此其全株及果实均为紫红色,为获取正常生长状态下紫色秋葵花青素合成代谢相关基因,采用Illumina HiSeq 2500高通量转录组测序技术对紫色秋葵全转录功能基因组测序后组装,再通过Nr、SwissProt和GO等三大数据库进行花青素相关功能基因筛选。三大数据库涉及35个花青素相关Unigene,具体包括19个花青素相关糖基转移酶、6个花青素酰基转移酶家族基因,5个无色花色素双加氧酶(合酶)以及5个花色素相关还原酶。这些花青素相关基因在Nr及SwissProt数据库中涉及较多相关植物,包括拟南芥、可可、树棉、木薯、苹果及葡萄等。聚类分析研究表明:涉及花青素相关四大家族成员的35个Unigene中,无色花色素双加氧酶亲缘关系最近,花青素酰基转移酶次之,花青素相关还原酶亲缘关系稍远,而花青素相关糖基转移酶家族成员亲缘关系最远。本研究获取的紫色秋葵众多花青素相关转移酶、合酶及还原酶等相关Unigene,为进一步研究紫色秋葵或其他植物的花青素合成代谢过程中基因克隆分析等奠定了坚实基础。

The rich content of anthocyanidin in purple Abelmoschus esculentus renders the characteristic color of the plant and its fruits.This study used the transcriptome of the plant to study the genetics associated with the anthocyanidin biosynthesis pathway under natural conditions.The transcriptome was sequenced using the Illumina HiSeq 2500 platform to isolate 35 anthocyanidin unigenes from the Nr,SwissProt and GO databases.These unigenes belonged to 4 distinct gene types including 19 of anthocyanidin-related glucosyltransferases,6 of anthocyanidin-related acyltransferases,5 of leucoanthocyanidin dioxygenases,and 5 of anthocyanidin-related reductases.The genes were annotated by Nr and SwissProt databases to show their associations with a variety of plants,such as Arabidopsis thaliana,Theobroma cacao,Gossypium arboretum, Manihot esculenta, Malus domestica and Vitis vinifera.A clustering analysis indicated that the unigenes had the closest relationship with the leucoanthocyanidin dioxygenase family,followed by the anthocyanin-related acyltransferases and the reductases,while the anthocyanidin-related glucosyltransferases showed the farthest homology.Identification of the 35 anthocyanidin-related unigenes would be useful for the gene cloning and analysis in studying anthocyanin biosynthesis of purple A.esculentus or other plants rich in anthocyanidin.