重组脂肪酶自诱导发酵培养基优化

Optimization of Auto-induction Fermentation Medium for Recombined Lipase

【目的】优化自动诱导发酵培养基,提高脂肪酶产量。【方法】通过对构建的一株转座子整合型脂肪酶重组菌株,采用自动诱导发酵表达方法,并结合CCD响应面实验设计方法,对自动诱导培养基中的碳源进行优化,获得了1个二次模型用于描述发酵培养基中碳源对产脂肪酶的影响。【结果】优化后的最适自动诱导培养基(W/V)组成为glycerol 2.596,glucose 0.035,lactose 1.289,tryptone 1.0,yeast extract 0.5,Na_2HPO_4·12H_2O 1.79,KH_2PO_4 0.68,NH_4Cl 0.267 5,Na_2SO_4 0.071,MgSO_4·7H_2O 0.05。【结论】发酵试验表明,最优碳源培养基的比活力为R_1=6.35U/mg,比初始发酵培养基提高近3倍。

[Objective]Optimizing the auto induction of the fermentation medium to increase lipase enzyme production. [Methods]In this research,through the construction of a transposon integrated lipase recombinant bacterial strain, using automatic induction fermentation expres sion method combined with CCD response surface experimental design method, the carbon source in the auto induction medium was optimized,and a quadratic model was obtained,which was used to describe the effect of the carbon source in the fermentation medium on lipase pro duction.[Results]The optimized fermentation medium （W/V） contained glycerol 2. 596,glu cose 0. 035,lactose 1. 289,tryptone 1.0,yeast extract 0. 5,Na2HPO4 ·12H2O 1. 79,KH2PO4 0. 68, NH4Cl 0. 267 5, Na2SO4 0. 071,MgSO4· 7H2O 0.05. [Conclusion]The results enzyme fermentation test confirmed that the lipase e specifi medium ly three times c activity in the optimized fermen was 6.35 U/mg,which was near higher than that in the initial fermentation medium.