锰浓度对不同结肠癌细胞株锰增强磁共振成像效果的影响

Impact of Manganese Concentration on Manganese-enhanced Magnetic Resonance Imaging in Different Colon Cancer Cell Lines

目的锰浓度是影响肿瘤锰增强磁共振成像(MEMRI)效果的最基本因素,本文研究其对不同结肠癌细胞株MEMRI效果的影响。材料与方法在0、0.05、0.10、0.20 mmol/L锰浓度下分别对人结肠癌细胞株SW620(高转移潜能)、SW480(低转移潜能)与正常细胞株CCD841 Co N进行体外MEMRI检测。T1 map计算细胞株在不同锰浓度下的平均T1缩短值。结果锰浓度为0.10、0.20 mmol/L时,3种细胞株平均T1缩短值大小依次为SW620>SW480>CCD841 Co N,差异有统计学意义(P<0.01)。正常细胞株在不同锰浓度(0.05、0.10、0.20 mmol/L)下平均T1缩短值差异无统计学意义(P>0.05);两种瘤细胞平均T1缩短值随锰浓度升高而升高,差异有统计学意义(P<0.01)。在较高锰浓度(0.10、0.20 mmol/L)条件下,SW620较SW480摄锰更多。结论结肠癌细胞体外摄锰程度随锰浓度升高而增加,MEMRI可区分具有不同转移潜能的结肠癌细胞株。

Purpose Manganese（Mn） concentration is one of the basic factors that have impact on manganese-enhanced magnetic resonance imaging（MEMRI） enhancement of neoplasm. This study aims to elucidate the impact of Mn concentration on enhancement of MEMRI in different colon cancer cells. Materials and Methods The human colon cancer cell SW620 with high metastatic potential, SW480 with low metastatic potential and the normal cell CCD841 Co N were performed MEMRI under different Mn concentrations（0, 0.05, 0.10 and 0.20 mmol/L） in vitro. The average values of T1 shortening were calculated by T1 map scan in different cells under different Mn concentrations. Results When the concentration of Mn was 0.10 and 0.20 mmol/L, the average value of T1 shortening of the three cell lines was SW620SW480CCD841 Co N（P〈0.01）. There was no significant difference in the average T1 value of the normal cell lines under different Mn concentrations（0.05, 0.10, 0.20 mmol/L）（P〈0.05）. The average T1 value of two tumor cells increased with the increase of Mn concentration. Under the condition of high Mn concentration（0.10 and 0.20 mmol/L）, SW620 took in more Mn than SW480. Conclusion Colon cancer cells increase Mn uptake with increasing of Mn concentration. MEMRI can distinguish colon cancer cells with different metastatic potential.