泰山白首乌、赤首乌及市售何首乌中游离大黄素和大黄素甲醚的比较研究

Comparative study of emodin and physcion in Radix cynanchi Auriculati,Fallopia multiflorum Thunb and marketed Fallopia multiflorum Thunb

目的采用高效液相色谱法测定白首乌、赤首乌以及市售何首乌中大黄素、大黄素甲醚的含量,旨在比较不同种类何首乌中两种成分含量的差异。方法采用大连依利特Sinochom ODS-BP色谱柱(4.6 mm×200 mm,5μm),以甲醇-0.1%磷酸溶液(90︰10)为流动相,检测波长254 nm,流速1.0 ml/min,进样量20μl,柱温25℃。结果大黄素、大黄素甲醚标准品在1.0~25μg/ml的浓度范围内,线性关系良好;泰山白首乌中未检测到大黄素及大黄素甲醚;赤首乌中大黄素和大黄素甲醚的含量分别为:33.49μg/g、35.21μg/g;市售何首乌中大黄素和大黄素甲醚的含量分别为88.03μg/g、33.43μg/g。结论本方法快速、准确,可用于何首乌中大黄素和大黄素甲醚的含量测定;不同种类何首乌中大黄素和大黄素甲醚的含量存在差异。

Objective： Determinating the content of emodin and physcion in different kinds of Fallopia multiflorum Thunb by HPLC method,aims to compare the difference. Methods： The analyses were performed on a Elite Sinochom ODSBP column（ 4. 6 mm × 250 mm,5 μm）,and the mobile phase was methanol-water containing 0. 1% phosphoric acid（ 90 ︰10） at the flow rate of 1 ml/min. The detection was carried out at 245 nm,the sample size is 20μl,and the column temperature was 25 ℃. Results： Emodinin and physcion in the concentration range of 1. 0 - 25 μg/ml,has a good linear relationship; The content of emodin and physcion in Fallopia multiflorum Thunb is 33. 49 μg/g,35. 21 μg/g; in the Fallopia multiflorum Thunb from market is 88. 03 μg/g,33. 43 μg/g; and in the radix cynanchi Auriculati does not detect the emodin and physcion. Conclusion： This content determination method is accruate,fast,easy to operate,has good stability,and can be used to be determine the content of emodin and Physcion; there has differences between different Fallopia multiflorum Thunb.