期刊文献+

叶酸修饰增强CdS-TiO_2光动力体外灭活HL60细胞效果探究 被引量:1

Effects of Folic Acid Modification Enhanced CdS-TiO_2 Photodynamic Inactivation of HL60 Cells in vitro
下载PDF
导出
摘要 本文研究了叶酸修饰硫化镉掺杂二氧化钛(FA-CdS-TiO_2)纳米颗粒体外光动力(PDT)灭活HL60细胞的作用效果,探讨了叶酸修饰增强CdS-TiO_2体外PDT效果的作用机理。采用表面修饰的方法制备FA-CdSTiO_2;通过透射电镜(TEM)、傅里叶红外光谱(FTIR)、荧光激发光谱(FS)方法,对纳米颗粒进行结构和光学性质的表征;采用CCK-8法检测细胞活性;利用荧光探针标记技术分析细胞内活性氧水平和细胞对纳米颗粒的摄取效率。实验结果表明:叶酸修饰后,纳米颗粒粒径大小和光学性质符合光敏剂要求,且不会引起新的细胞毒性;通过叶酸修饰,细胞对纳米颗粒的摄取效率提升,细胞内活性氧产量提高,FA-CdS-TiO_2纳米颗粒的PDT效率明显增强,在FA-CdS-TiO_2浓度为20μg/m L时,暗室细胞存活率约为85%,可见光下对HL60细胞的灭活率达78%,实现了较低暗毒性下的较高PDT灭活效率。 The photocatalytic inactivation efficiency of HL60 cells in vitro based on cadmium sulfide-doped titanium dioxide modified folic acid( FA-CdS-TiO2) and the mechanism of folic acid enhancing the effect of CdS-TiO2 on PDT were researched in this paper. The FA-CdS-TiO2 nanoparticles were prepared by surface modification method. The nanoparticles were characterized using transmission electron microscopy( TEM),fourier transform infrared spectroscopy( FTIR),fluorescence excitation spectroscopy( FS). Cell viability was measured by CCK-8 method. The intracellular reactive oxygen species( ROS) and the uptake efficiency of nanoparticles were analyzed by fluorescence probe labeling technique.The results showed that the size and optical properties of nanoparticles after folic acid modification met the requirements of photosensitizer,and the naoparticles don't cause new cytotoxicity. After folic acid modification,the uptake efficiency of nanoparticles by cells was enhanced,the production of ROS in cells increased,and the PDT efficiency of FA-CdSTiO2 nanoparticles increased significantly. When the concentration of FA-CdS-TiO2 was 20 μg/m L,the cell survival rate is about 85% under darkroom conditions,the inactivation rate reached 78% under visible light,which indicates that the higher PDT inactivation efficiency under lower dark toxicity is achieved.
作者 孙广宇 张卿 熊建文 SUN Guangyu;ZHANG Qing;XIONG Jianwen(Guangdong Provincial Key Laboratory of Quantum Engineering and Quantum Materials,School of Physics and Teleeommunieation Engineering,South China Normal University,Guangzhou 510006,Guangdong,China;State Key Laboratory-of Advanced Optical Communieation Systems and Networks,Key Laboratory for Laser Plasmas(Ministry of Education),Department of Physics and Astronomy,Shanghai Jiao Tong University,Shanghai 200240,China)
出处 《激光生物学报》 CAS 2018年第1期21-27,共7页 Acta Laser Biology Sinica
基金 国家自然科学基金(61072029) 广州市科技计划项目(2014J4100049)
关键词 叶酸 纳米FA-CdS-TiO2 光动力疗法 HL60细胞 荧光 folic acid nano-FA-CdS-TiO2 photodynamic therapy HL60 cells fluorescence
  • 相关文献

参考文献6

二级参考文献39

共引文献191

同被引文献3

引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部