雷公藤红素对小鼠腹腔巨噬细胞极化分型的影响

Impact of celastrol on polarization of mouse peritoneal macrophage

目的研究雷公藤红素(Cel)对小鼠腹腔巨噬细胞极化分型的影响。方法腹腔灌肉汤法(刺激法)分离出小鼠腹腔巨噬细胞,将细胞分为对照组(con)、单纯雷公藤红素干预组(Cel)、经典激活M1组(IFN-γ+LPS)和雷公藤红素干预M1组(IFN-γ+LPS+Cel),用real-time PCR、流式计量术分析检测巨噬细胞两种极化分型(M1型和M2型)相关标志物诱导型一氧化氮合酶(i NOS)、精氨酸酶1(Arg-1)、甘露糖受体(MR或CD206)和补体受体4(CR4或CD11c)的表达。结果雷公藤红素引起M1型巨噬细胞相应标志物CD11c、i NOS表达减少(P<0.05),或使M2型巨噬细胞相应标志物Arg-1表达增加(P<0.05)。结论雷公藤红素能抑制小鼠腹腔巨噬细胞向M1型极化。

Objective To explore the impact of celastrol on polarization of mouse peritoneal macrophage. Methods Mouse peritoneal macrophages were isolated by intraperitoneal injection of broth（ stimulation method）. Cells were divided into control group,celastrol only group,classically activated M1 group and celastrol intervention with M1 group. RT-q PCR and flow cytometry were used to detect the relevant markers（ i NOS,Arg-1,CD206,CD11 c） expression levels of two polarization states of macrophages（ M1 macrophages and M2 macrophages）. Results Celastrol could decrease the expressions of revelant markers CD11 c and i NOS of M1 macrophages（ P〈0. 05）. Celastrol could enhance the expressions of revelant marker Arg-1 of M2 macrophages（ P〈0.05）. Conclusions Celastrol can suppress M1 macrophage polarization of mouse peritoneal macrophages.