纳米铜硅胶膜厚度对MAS-IUS控释效果影响的实验研究

Experiment study on the effect of thickness sheath of nano-copper-silica gel on the release of levonorgestrel in MAS-IUS

目的研究不同厚度纳米铜硅胶膜对记忆合金硅胶宫内缓释系统[Memory Alloy Silicone Intrauterine System,MAS-IUS]中左炔诺孕酮(LNG)释放量造成的影响,并探讨其释放模式。方法将直径为0.3 mm的钛镍合金丝在280℃下盘旋形成外径为3 mm的合金丝管架;将一定比例(质量比)的纳米铜硅胶混合物封闭合金丝管架外壁,制成膜厚度分别为0.5、1.0、1.5、2.0 mm的"钛镍记忆合金纳米酮硅胶管"模型。将52 mg LNG置于管腔中,以硅胶粘合剂封堵两端,制成不同纳米铜硅胶膜厚度的MAS-IUS。将不同膜厚度的MAS-IUS模型置于盛有10 m L无水乙醇的离心管中,置于在37℃恒温水浴箱中,定期用紫外分光光度计测量离心管中LNG含量。结果一定剂量的LNG在MAS-IUS模型中的释放速率随纳米铜硅胶膜的增厚而下降,各组释放量均在5 d后趋于稳定,表现为在一定范围内波动的缓释规律。结论 LNG在不同纳米铜硅胶膜厚度的MAS-IUS中,表现为先爆释、然后缓释的状态;可以通过调整纳米铜硅胶膜厚度控制MAS-IUS中LNG的释放量。

Objective Investigate the effects of different thickness of nano-copper-silica gel on the release of levonorgestrel（ LNG） in Memory Alloy Silicone Intrauterine System（ MAS-IUS）,and further explore its release pattern. Methods The Ti-Ni alloy wire（ diameter of 0. 3 mm） was spirally formed at 280 ° C as an alloy wire tube with 3 mm outer diameter. A mixture of nano-copper-silica gel with certain ratio（ mass ratio） was used to close the outer wall of the alloy wire tube frame. Therefore,the different thickness sheath model with 0. 5,1. 0,1. 5 and 2. 0 mm ＂ titanium nickel memory alloy nano silicone tube＂ was prepared. LNG（ 52 mg） was placed in the tube and both ends of the tube were sealed with a silicone adhesive. The MAS-IUS with different thickness sheath was immerged in a centrifuge tubes containing 10 ml of absolute ethanol and then the tubes were placed in a thermostatic water bath at 37°C. The amount of LNG in the centrifuge tube was measured periodically with a UV spectrophotometer. Results LNG release rate in these MAS-IUS models was decreased with the thickness of controlling sheath. LNG release in each group tended to be stable after 5 days with the law of sustained release and fluctuating in a certain range. Conclusion LNG in MAS-IUS is manifested as the manner of first burst release and then slow release. The release of LNG in MAS-IUS could be controlled by adjusting the thickness sheath of nano-copper-silica gel.