人参皂苷Rb2对高脂性脂肪肝小鼠肝脏脂质代谢的影响及其机制

The effect of ginsenoside Rb2 on the regulation of lipid metabolism in fatty liver mice and its mechanism

目的:探讨人参皂苷Rb2对高脂性脂肪肝小鼠肝脏脂质代谢的影响及其机制。方法:雄性C57BL/6J小鼠18只,随机分为正常对照组、高脂模型组和人参皂苷Rb2组。正常对照组用10%kcal对照饲料喂养,其余用60%kcal高脂饲料喂养8周,按分组情况分别予PBS或人参皂苷Rb2(40 mg·kg^(-1)·d-1)腹腔注射10 d。HE染色观察脂肪变性程度,荧光定量PCR检测肝脏脂肪代谢相关基因的表达,荧光定量PCR和Western blot检测肝脏过氧化物酶体增殖物激活受体(PPAR)α表达。结果:人参皂苷Rb2明显减轻肝质量,改善肝脂肪变性,下调脂肪酸合成酶(FAS)、脂蛋白脂酶(LPL)、固醇调节元件结合蛋白(SREBP)-1c和上调肉碱棕榈酰转移酶-1(CPT-1)、胆固醇7α羟化酶(CYP7A1)的mRNA水平,增加PPARα基因和蛋白的表达。结论:人参皂苷Rb2可以改善小鼠脂质代谢,其作用机制可能与增加肝中PPARα表达,调节脂代谢相关靶基因SREBP-1c、FAS和CYP7A1表达有关。

Objective： To investigate the role of ginsenoside Rb2 in hepatic lipid metabolism in fatty liver mice and its mechanism. Methods： Male C57 BL/6 J mice were randomly divided into normal control group, high-fat model group and ginsenoside Rb2 group. The normal control group was fed with 10% kcal control diet, and the rest was fed with 60% kcal high fat diet for 8 weeks. PBS or ginsenoside Rb2（40 mg·kg-1·d-1） was injected intraperitoneally for 10 days. Realtime PCR and Western blot were used to investigate the expression and mechanism of lipid metabolism-related genes such as lipid synthesis and fatty acid oxidation in liver tissues. Results： Ginsenoside Rb2 significantly reduced liver weight, improved hepatic steatosis, down-regulated fatty acid synthase（FAS）, lipoprotein lipase（LPL）, sterol regulatory element binding protein（SREBP）-1 c, and up-regulated carnitine palmitoyl transferase（CPT）-1 and cholesterol 7α-hydroxylase（CYP7 A1） m RNA levels, increased the expression of hepatic peroxisome proliferator-activated receptor（PPAR） alpha gene and protein. Conclusion： Ginsenoside Rb2 can attenuate hyperlipidemic fatty liver, and its mechanism may be related to the increase of PPARα expression in the liver and the regulation of target genes expression such as SREBP-1 c, FAS and CYP7 A1 related to lipid metabolism.