PI3Kp85α在甲状腺乳头状癌中的表达及意义

The expression and signifi cance of PI3Kp85α in papillary thyroid carcinoma

目的:探讨PI3Kp85α在甲状腺乳头状癌组织中的表达及其临床意义。方法:采用免疫组织化学法、Western blot和ELISA法检测116例甲状腺乳头状癌组织、90例甲状腺乳头状增生组织中PI3Kp85α蛋白的表达情况,并分析蛋白表达与患者性别、年龄、肿瘤大小、淋巴结转移等临床病理特征的相关性。利用ROC曲线评价PI3Kp85α在甲状腺乳头状癌诊断中的价值。结果:PI3Kp85α蛋白的阳性表达率在甲状腺乳头状癌组织中显著高于甲状腺乳头状增生组织(69.8%vs.31.1%),2组间差异有统计学意义(P<0.05)。甲状腺乳头状癌中,PI3Kp85α蛋白表达与淋巴结转移、TNM分期相关(P<0.05);PI3Kp85α诊断甲状腺乳头状癌的ROC曲线下面积为0.966,当cut-off值取2.100时,诊断灵敏度(为92.2%)和特异度(为91.1%)较高。结论:甲状腺乳头状癌组织中PI3Kp85α蛋白的阳性表达率明显升高,且其表达水平与淋巴结转移和肿瘤分期相关,可能作为评估甲状腺乳头状癌侵袭性及预后的指标。

Objective： To explore the expression and significance of phosphatidylinositol 3-kinase regulatory subunit alpha（PI3 Kp85α） in papillary thyroid carcinoma（PTC）. Methods： PI3 Kp85α expressions were detected in PTC tissues（n=116） and thyroid papillary hyperplasia（PTH） tissues（n=90） by immunohistochemistry, Western blot, and enzyme-linked immunosorbent assay. The relationships between PI3 Kp85α expressions and clinical pathological features in PTC were analyzed. Diagnostic values of PI3 Kp85α in PTC were evaluated by receiver operating characteristic（ROC） curve. Results： PI3 Kp85α expression levels in PTC tissues were statistically higher than those in PTH tissues（P〈0.05）. PI3 Kp85α expression was associated with lymph node metastasis（P〈0.05）. The diagnostic sensitivity and specificity of PI3 Kp85α were 92.2% and 91.1%, respectively, with a cut-off value of 2.100 and an area under curve of 0.966. Conclusion： PI3 Kp85α may be related to the tumorigenesis and progression of PTC.