thanatin串联抗菌肽原核表达研究

Expression of antimicrobial peptides thanatin tandem fusion in Escherichia coli

抗菌肽thanatin是一类广谱性的阳离子抗菌活性肽,对病原性真菌有很好的杀灭效果。为了提高thanatin抗菌肽表达丰度,将3个thanatin单体拷贝基因进行串联,与MBP蛋白进行融合表达,表达的产物经过酶切纯化后,通过Western blot和质谱鉴定,结果表明3×thanatin在大肠埃希菌中成功表达,对核盘菌的抑菌活性测定结果表明,原核表达的3×thanatin最低抑菌浓度为6.6μmol·L^(-1),而化学合成的thanatin抗菌肽对核盘菌的抑菌浓度约为64μmol·L^(-1),3×thanatin抗菌肽对核盘菌的抑菌活性提升了约1个数量级。

The antibacterial peptide thanatin is a broad spectrum cationic antibacterial peptide,which can significantly kill pathogenic fungi.In order to improve the expression level of thanatin,three copies of thanatin monomer and MBP protein was co-expressed in Escherichia coli.The expression products were identified by Western blot and mass spectrometry after enzyme digestion and purification.The results showed that the three copies of thanatin fusion protein,3×thanatin,were successfully expressed in E.coli and had good inhibition activity on Sclerotinia sclerotiorum.The minimum concentration of inhibition on S.sclerotiorum was 6.6μmol·L-1for 3×thanatin,which was about ten times higher than 1×thanatin.