摘要
采用内部沸腾法优化螺旋藻多糖提取工艺.利用正交实验设计分析乙醇浓度、料液比、提取温度和时间对螺旋藻多糖得率的影响.结果表明:用内部沸腾法提取螺旋藻多糖的最佳条件为:40%乙醇,料液比1∶14,温度80℃,提取时间5min,多糖得率是8.92%.内部沸腾法快速、高效、经济,可有效用于螺旋藻多糖的提取.
Polysaccharides from Spirulina Platensis were extracted by inner ebullition method.The effects of parmeters ranged?from concentration of ethanol,solid-liquid ratio,extraction temperature,and time on the yield of Polysaccharides were investigated by orthogonal experiment.Experiment results show that the optimum extraction conditions are as followed:the volume fraction of ethanol solution saturated the materials is 40%,the ratio of material to solution was 1:14(g/mL),the extraction temperature was 80℃,extraction time was 5 min.The extraction rate of polysaccharides reached 8.92% under the optimum extraction.The method of inner ebullition is a rapid,efficient,economic method to extract the polysaccharide fromSpirulina Platensis.
作者
刘玉环
贺亚银
张喜峰
张丽妹
LIU Yu-huan;HE Ya-yin;ZHANG Xi-feng;ZHANG Li-mei(College of Agriculture and Biotechnology Hexi University,Zhangye 734000,China;Key Laboratory of Hexi Corridor Resources Utilization of Gansu,Zhangye 734000,China)
出处
《牡丹江师范学院学报(自然科学版)》
2018年第2期51-54,57,共5页
Journal of Mudanjiang Normal University:Natural Sciences Edition
基金
甘肃省重点研发计划项目(17YF1NG050)
关键词
螺旋藻
内部沸腾法
多糖
Spirulina Platensis
inner ebullition method
polysaccharides