口腔鳞状细胞癌患者唾液和血清中N-α-乙酰基转移酶10的水平及其临床意义

Level of N-α-acetyltransferase 10 in saliva and serum of patients with oral squamous cell carcinoma and its clinical significance

目的探讨N-α-乙酰基转移酶10(Naa10)在口腔鳞状细胞癌患者和正常人的唾液和血清中的水平及其临床意义。方法应用酶联免疫吸附测定(ELISA)法检测口腔鳞状细胞癌患者和正常人的唾液和血清中Naa10的水平,并分析Naa10水平与口腔鳞状细胞癌患者年龄、性别、族别、分化程度、临床分期、肿瘤转移等临床参数的相关性,绘制接受者操作特征(ROC)曲线评价唾液、血清中Naa10水平在口腔鳞状细胞癌诊断中的应用价值。结果口腔鳞状细胞癌患者的唾液和血清中Naa10水平均高于正常对照组。无论是口腔鳞状细胞癌患者还是正常人,唾液中Naa10水平都高于血清中。口腔鳞状细胞癌患者血清中Naa10水平在不同年龄组的差异具有统计学意义(P<0.05),唾液中Naa10水平在不同分化程度组的差异具有统计学意义(P<0.05),其余差异无统计学意义。通过ROC曲线评价唾液和血清中Naa10水平,其ROC曲线下面积分别为0.924、0.713,此时两组灵敏度、特异度和准确度分别为85.4%、85.0%、85.3%和74.2%、65.0%、72.1%。结论检测唾液和血清中的Naa10水平可用于协助诊断口腔鳞状细胞癌,且其在唾液中的灵敏度和特异度更优于血清。唾液中Naa10水平与分化程度相关,可能为判断口腔鳞状细胞癌恶性程度提供帮助。

Objective To detect the level of N-a-acetyltransferase 10 （Naa10） in the saliva and serum of patients with oral squamous cell carcinoma （OSCC） and normal adults, and to explore its clinical significance. Methods Using enzyme- linked immunosorbent assay （ELISA） to detect the levels of Naa10 in the serum and saliva of OSCC patients and normal adults, the correlation between the level of Naa10 and age, gender, nationality, histological grade, clinical stage and tumor metastasis were analyzed. To evaluate the value of salivary and serum Naa10 in the diagnosis of OSCC by receiver operating characteristic （ROC） curve. Results The levels of Naa10 in saliva and serum of patients with OSCC were higher than those in normal controls. In both patients with OSCC and normal controls, salivary Naa10 was higher than serum Naa10. Level of Naa10 in serum was correlated with the age of OSCC patients （P〈0.05）. Level of Naa10 in saliva was correlated with the histological grade of OSCC patients （P〈0.05）. But there was no statistical difference between the other pathological parameters and Naa10 levels in saliva and serum. The area under the ROC curve of Naa10 in saliva and serum was 0.924 and 0.713. In saliva and serum, the sensitivity, specificity and accuracy of Naa10 were 85.4%, 85.0%, 85.3% and 74.2%, 65.0%, 72.1%. Conclusion Detection of Naa10 levels in saliva and serum is useful in diagnosis of OSCC, and its sensitivity and specificity in saliva are better than those in serum. The level of Naa10 in saliva is related to the degree of differentiation, which may help to determine the degree of malignancy of OSCC