用于快速识别红耳龟在中国野外分布的特异性引物筛选

Specific primers screening for rapid identification of alien red-eared slider(Trachemys scripta elegans) in the wild of China

红耳龟(Trachemys scripta elegans)是世界上最危险的100个入侵物种之一,自20世纪80年代被引入中国,目前已在中国野外广泛分布。环境DNA技术是物种调查和监测的有力工具,为了能够利用环境DNA技术快速识别中国野外红耳龟的分布状况,筛选了红耳龟线粒体COI基因的特异性引物。该引物能够有效区分红耳龟与中国野外其他常见的10种龟类,可为红耳龟的野外分布监测和防控提供参考。

The red-eared slider（ Trachemys scripta elegans）,one of the world＇s top 100 worst invasive alien species,was introduced to China in 1980 s. It is now widely distributed in the wild of China. Environmental DNA technology is a powerful tool for detection and monitoring of wildlife. In order to develop environmental DNA technology for the investigation of red-eared sliders in China,this study screened specific primers for the mt DNA COI gene of red-eared slider. This specific primer can be used to quickly identify redeared slider from the other 10 common turtle species found in the wild of China and therefore provides technical reference for field monitoring and control of the red-eared sliders.