摘要
目的:探讨加味七方胃痛颗粒对裸鼠原位移植胃癌三叶因子3(trefoil factor family3,TFF3)的表达及其细胞外信号调节激酶(extracellular signal-regulated kinase,ERK)/核因子кB(nuclear factor-кB,NF-кB)信号通路的调控机制。方法:复制裸鼠胃癌原位移植模型,随机分成正常组(即空白对照组),氟尿嘧啶(5-fluorouracil,5-FU)组,5-FU+加味七方胃痛颗粒组,加味七方胃痛颗粒组,每组15只。采用Western blot、RT-PCR检测加味七方胃痛颗粒对裸鼠原位移植胃癌中TFF3、NF-кB、ERK基因及蛋白表达的影响。结果:加味七方胃痛颗粒组TFF3、NF-кB、ERK m RAN及蛋白表达量较正常组降低(P〈0.05),5-FU+加味七方胃痛颗粒组TFF3、NF-кB、ERK m RAN及蛋白表达量较5-FU组降低(P〈0.05)。结论:加味七方胃痛颗粒可能通过抑制ERK/NF-кB信号通路参与下调裸鼠原位移植胃癌TFF3、NF-кB、ERK表达水平,从而达到抗癌作用。
Objective:To investigate the Jiawei Qifang Weitong(JWQFWT)Granules on orthotopic gastric cancer trefoil factor family3(trefoil factor family3,TFF3)and the expression of extracellular signal regulated kinase/nuclear factor-κB regulation signal pathway. Methods:The nude mice model of orthotopic transplantation of gastric cancer were randomly divided into blank control group,fluorouracil group,5-FU+ modified JWQFWT Granules group,supplemented by the JWQFWT Granules group,each group of 15. To calculate the orthotopic transplantation of human gastric cancer in nude mice tumor inhibition rate by Western,blot,RT-PCR detection of modified JWQFWT Granules on TFF3 orthotopic gastric cancer,NF-κB,the expression of ERK gene and protein. Results:The modified JWQFWT Granules group TFF3,NF-κB,expression of ERK m RAN and protein were lower than those of blank control group(P〈0.05),5-FU+ with JWQFWT Granules group TFF3,NF-κB,expression of ERK protein and m RAN was lower than group 5-FU(P〈0.05). Conclusion:The prescription of the JWQFWT Granules can effectively inhibit ERK/NF-κB signaling pathway involved in the down-regulation of orthotopic gastric cancer TFF3,NF-κB,ERK expression level,so as to achieve the anti-cancer effect.
作者
谢卓恩
陈国忠
黄丽萍
苏洪佳
刘坤荣
彭鸿
方丽娇
彭振西
XIE Zhuoen;CHEN Guozhong;HUANG Liping;SU Hongjia;LIU Kunrong;PENG Hong;FANG Lijiao;PENG Zhenxi(Guangxi University of Chinese Medicine,Nanning 530001,Guangxi,China;First Affilialed Hospital of Guangxi University of Chinese Medicine,Nanning 530023,Guangxi,China)
出处
《辽宁中医药大学学报》
CAS
2018年第8期26-29,共4页
Journal of Liaoning University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81260529)
关键词
胃癌
原位移植
加味七方胃病颗粒
三叶因子3
ERK/NF-кB信号通路
gastric cancer
orthotopic transplantation
Jiawei Qifang IVeitong Granules
trefoil tiaetorfamily 3
ERK/NF-κB signaling pathway