紫薯黄酮对酒精诱导人正常肝细胞氧化损伤保护作用

Protective Effective of Purple Sweet Potato Flavones on Ethanol-induced Damaged HL7702

目的：研究紫薯黄酮对酒精损伤人正常肝细胞（HL7702）的保护作用。方法：分别以酒精培养（摩尔浓度100、200、300、400、500、600、700、800 mm）HL7702,CCK-8检测细胞的生存率以筛查复制模型的最佳浓度以及考察紫薯黄酮对细胞的毒性的影响。并使用试剂盒比色法检测各组细胞超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-PX）、过氧化氢（H2O2）及总抗氧化能力（T-AOC）水平。采用统计分析软件SPSS17.0进行统计学分析。结果：建立酒精诱导损伤模型最佳浓度为500 mm;紫薯黄酮最适宜的给药范围是100~800μg/m L;200、400、600、800μg/m L的紫薯黄酮可以剂量依赖性的升高酒精损伤模型中HL7702的存活率,并提高HL7702细胞内的T-AOC、SOD、CAT及GSH-PX水平,同时降低H2O2的生成。结论：紫薯黄酮能够保护酒损伤的HL7702,其保护机制与增强T-AOC、SOD、CAT及GSH-PX活性,降低H2O2含量有关。

Objective：To expore the safe dose range of purple sweet potato flavones（PSPF）in HL7702,the protective the effect of PSPF in alcohol damaged models. Methods：HL7702 were cultured with alcohol（100,200,300,400,500,600,700,800 mm）,the activity of cells was tested by CCK-8 assay to screen optimal concentration of model and to study influence of PSPF on HL7702 toxicity and to evaluate the levels of SOD,CAT,GSH-PX,H2 O2 and T-AOC in different controls. Statistic was performed with SPSS 17.0. Results：For inducing model,the optimal concentration of alcohol was 500 mm,and that of PSPF was 100-800 μg/m L;Pretreatment of HL7702 cells with 200,400,600 or 800 μg/m L PSPF dose dependently improved the cell viability in the alcohol-induced model;enhanced the levels of T-AOC,SOD,CAT,GSH-PX and decreased H2 O2 levels. Conclusion：PSPF can protect the alcohol-induced cytotoxicity model HL7702,and the protective mechanism would be associated with the improvement of T-AOC,SOD,CAT,GSH-PX activity,the reduction of H2 O2 levels.