黄柏苷对黄檗种子休眠程度影响的初步研究

Phellamurin:Effect on the Dormancy of Phellodendron amurense Rupr. Seeds

为研究黄柏苷与环境温度、种子休眠程度的相关性,探讨黄柏苷在温度信号介导中可能存在的作用。以高效液相法检测不同生长地及年度黄檗叶片中的黄柏苷含量,滤纸培养法检测黄檗种子发芽率确定其休眠程度。持续监测生长地气温。通过在黄檗繁殖器官上喷施黄柏苷,考察黄柏苷对种子休眠程度的直接影响。结果表明,生长地花期均温相差5℃,黄檗叶片中黄柏苷含量均值相差54.46 mg/g,层积30天种子发芽率相差40.45%;不同年度间花期均温相差1.16℃,黄柏苷含量均值相差4.83 mg/g,层积30天种子发芽率相差23.07%;喷施黄柏苷,可使种子休眠程度显著加深,种子发芽率降低57.34%。研究结果提示三者之间存在环境温度越低,黄檗叶片中黄柏苷含量越高,黄檗种子休眠程度越深的关联,温度对黄檗种子休眠程度的调控可能是通过调节黄柏苷含量,经过某种体内途径实现的。

This study aims at studying the correlation between phellamurin and the environment temperatureand seed dormancy degree, exploring the role of phellamurin in temperature signal mediation. Highperformance liquid chromatography（HPLC） is used to measure phellamurin content in leaves of Phellodendron amurense Rupr. at different locations and different years. The filter paper culture method was taken to measurethe seed germination rate to determine the dormancy degree. Besides the temperature of sample sites wereconstantly monitored by temperature recorder. Through spraying phellamurin on the reproductive organs of Phellodendron amurense Rupr., the direct effect on seed dormancy degree by phellamurin was examined.Results showed that there was a difference of 5℃ in average temperature at flowering stages of Phellodendron amurense Rupr. within their growth areas, a difference of 54.46 mg/g in the average phellamurin contents in Phellodendron amurense Rupr. leaves, and a difference of 40.45% in the germination rates of seeds stratifiedfor 30 days. By contrast, in different years, there was a difference of 1.16℃ in average temperature at floweringstages, a difference of 4.83 mg/g in the average phellamurin content, and a difference of 23.07% in thegermination rate of seeds stratified for 30 days. By spraying phellamurin, the degree of seeds dormancydramatically intensified and the germination rate decreased by 57.34%. The results suggested that the lower the environment temperature, the higher phellamurin content in leaves of Phellodendron amurense Rupr., andthe deeper degree of seeds dormancy would be. The way of temperature regulating seeds dormancy degree of Phellodendron amurense Rupr. might be achieved by adjusting phellamurin content through some internalapproaches.