犬细小病毒YBYJ株体外诱导MDCK宿主细胞凋亡的研究

Study on apoptosis of MDCK host cells induced by canine parvovirus YBYJ strain in vitro

为研究犬细小病毒(CPV)在体外能否诱导MDCK宿主细胞凋亡,本研究应用前期分离到的CPV YBYJ强毒株接种MDCK细胞,通过倒置显微镜观察CPV导致MDCK宿主细胞病变(CPE)情况,采用细胞凋亡DNA Ladder抽提试剂盒和Annexin V-FITC/PI双染细胞凋亡检测试剂盒和Caspase-3活性检测试剂盒检测,观察CPV诱导MDCK宿主细胞凋亡情况。结果显示,CPV YBYJ强毒株可导致MDCK宿主细胞产生明显CPE,并出现明显的DNA Ladder,Annexin V-FITC/PI双染为阳性,Caspase-3活性显著升高,提示CPV在体外能够诱导MDCK宿主细胞凋亡。

In order to study the effects of canine parvovirus （CPV） inducing apoptosis in MDCK host cells in vitro, MDCK cells were inoculated with virulent CPV YBYJ strain isolated from the previous study, which induced cytopathic effect （CPE） in MDCK cells. Then the apoptosis in the virus infected MDCK cells was examined by ladder DNA extraction kit, Annexin V-FITC/PI double staining cell apoptosis detection kit and Caspase-3 activity detection kit. The results showed that CPV was able to cause the MDCK host cell to appear obvious ladder DNA, positive for Annexin V-FITC/PI double staining, significant increase of Caspase-3 activity. It suggested that CPV had the ability to induce the apoptosis of MDCK host cells in vitro.