长链非编码RNA RP11-214F16.8促进乳腺癌细胞增殖

Long Non-coding RNA RP11-214F16.8 Enhances Proliferation of Breast Cancer Cells

长链非编码RNAs(long non-coding RNAs,lncRNAs)在乳腺癌发生发展中的作用备受瞩目。本研究通过大数据分析发现,lncRNA RP11-214F16.8在乳腺癌组织中的表达量显著高于正常组织,且其表达量与乳腺癌患者预后负相关。因此,本文采用qRT-PCR技术,在收集的临床样本中验证RP11-214F16.8的表达,证实其在乳腺癌组织中相对表达量为5.65±0.72,其在癌旁组织中表达量为2.63±0.35,且RP11-214F16.8的表达量与乳腺癌瘤体大小和临床TNM分期相关。此外发现,RP11-214F16.8在乳腺癌细胞中的表达量高于正常乳腺上皮细胞。在乳腺癌MCF-7细胞中,过表达RP11-214F16.8后,MCF-7细胞的增殖能力显著增强。而在MDA-MB-231细胞中,敲低RP11-214F16.8的表达,获得相反的结果。进一步研究发现,RP11-214F16.8可上调细胞周期蛋白D3、CDK4的表达,而下调p18蛋白表达量,进而加速细胞增殖。总之,RP11-214F16.8在乳腺癌中高表达,且促进乳腺癌细胞增殖,进而推动乳腺癌进程。这一研究发现,将为乳腺癌发生发展的网络机制研究提供新的理论依据。

Long non-coding RNAs（lncRNAs） were highlighted in the progression of breast cancer. In this study,it was found that RP11-214 F16. 8 was significantly enhanced in breast cancer tissues in data set analysis by GEPIA software, comparing with that in normal. Moreover, RP11-214 F16. 8 was negatively correlated with the prognosis of breast cancer patients. Furthermore,qRT-PCR assay was performed to determine the expression levels of RP11-214 F16. 8 in breast cancer and normal tissues collected at our hospital. The results showed that RP11-214 F16. 8 was highly expressed in breast cancer tissues（5. 65 ± 0. 72） than that in normal tissues（2. 63 ± 0. 35）. RP11-214 F16. 8 expression was positively correlated with tumor size and TNM stage of breast cancer. Similarly,it was demonstrated that RP11-214 F16. 8 was evidently accelerated in breast cancer cells,comparing with that in mammary MCF-10 A epithelial cells. Restoring RP11-214 F16. 8 enhanced proliferation of MCF-7 cells,while silencing RP11-214 F16. 8 reduced MDA-MB-231 cells proliferation. RP11-214 F16. 8 could upregulate cyclin D3 and CDK4 protein expression while downregulated p18 protein expression. To sum up,RP11-214 F16. 8 was highly expressed in breast cancer and enhanced proliferation of breast cancer cells. This investigation can provide new insight into the molecular mechanisms of breast cancer progression.