靶向干扰Livin对HEC-1-A细胞侵袭迁移能力的影响

Effects of target Livin interference on invasion and migration ability of HEC-1-A cells

目的探讨靶向抑制Livin对人子宫内膜癌细胞株(HEC-1-A)细胞侵袭及迁移能力的影响。方法将Livin的特异性小分子干扰RNA(Livin-siRNA)和阴性对照序列(Livin-NC)应用脂质体法转染HEC-1-A细胞,试验分为Livin-si组、Livin-NC组和Livin-B组。应用qRT-PCR及Western blot法检测转染LivinsiRNA后,Livin mRNA及蛋白表达情况。Transwell小室试验观察干扰Livin后,HEC-1-A细胞侵袭及迁移能力的变化。结果 qRT-PCR和Western blot结果显示,Livin-si组Livin mRNA及蛋白相对表达量明显减少(P<0.01)。侵袭及迁移试验结果显示,抑制Livin表达后,Livin-si组穿膜细胞数明显减少(P<0.01)。结论Livin-siRNA能有效抑制Livin在HEC-1-A细胞中的表达,靶向干扰Livin可明显减弱HEC-1-A细胞的侵袭及迁移能力。

Objective To investigate the effect of targeted inhibition of Livin on the invasion and migration of endometrial carcinoma HEC-1-A cells.Methods Livin-specific small interfering RNA（Livin-siRNA）and negative control sequence（Livin-NC）were transfected into HEC-1-A cells by liposome method.The experiment was divided into Livin-si group,Livin-NC group and Livin-B group.The expression of Livin mRNA and protein was detected by qRT-PCR and Western blot after transfection of Livin-siRNA.Transwell chamber was used to observe the changes of cell invasion and migration after Livin interference.Results qRT-PCR and Western blot results showed that the expression level of Livin mRNA and protein in Livin-si group was significantly decreased（P〈0.01）.The invasion and migration experiment results showed that the number of transmembrane cells after inhibiting Livin expression in the Livin-si group was significantly decreased（P〈0.01）.Conclusion Livin-siRNA can effectively inhibit the expression of Livin in HEC-1-A cells.Target-interfering Livin can significantly weaken the invasion and migration ability of HEC-1-A cells.