间质性肺疾病患者外周血双阴性T淋巴细胞的变化及意义

Change and significance of peripheral blood double-negative T-lymphocytes in patients with interstitial lung disease

目的研究间质性肺疾病中外周血双阴性T淋巴细胞(DNT)的变化及其临床意义。方法收集该院86例间质性肺疾病患者(病例组)的临床资料(结缔组织病相关性间质性肺疾病37例,特发性肺间质纤维化36例,其他类型间质性肺疾病13例),选取同期健康人群45例作为对照组。收集研究对象的外周血标本,利用FC500流式细胞仪检测DNT细胞的表达水平。结果病例组DNT细胞表达水平明显低于对照组(t=4.194,P<0.01)。其中,特发性肺间质纤维化患者(t=-4.790,P<0.01)及结缔组织病相关间质性肺疾病患者(t=-4.321,P<0.01)的DNT表达水平与对照组相比差异有统计学意义。结缔组织病相关性间质性肺病患者与特发性肺间质纤维化患者的外周血DNT表达水平差异无统计学意义(t=-0.065,P=0.948)。结论DNT水平低表达在间质性肺疾病中可能起到一定病理生理作用。

Objective To investigate the changes and clinical significance of peripheral blood doublenegative T-lymphocytes（DNT）in interstitial lung disease（ILD）.Methods The clinical data in 86 cases of ILD（case group）treated in this hospital from April 2015 to November 2017 were collected,including 37 cases of connective tissue disease related ILD,36 cases of idiopathic pulmonary fibrosis（IPF）and 13 cases of other types of ILD.Forty-five healthy people in corresponding period were selected as the control group.The peripheral blood samples in the research subjects were collected and expression level of ONT cells was detected by FC500 flow cqtometry.Results The expression level of DNT in the case group was significantly lower than that in the control group（t=4.194,P〈0.01）.Among them,the expression level of DNT in the IPF group and connective tissue disease related ILD group had statistical difference compared with that in the control group（t=-4.790,-4.321;P〈0.01）.The expression level of peripheral blood DNT lympnocytes had no statistical difference between the connective tissue disease related ILD and IPF（t=-0.065,P=0.948）.Conclusion The low expression of DNT lymphocytes may play a certain pathophysiological role in ILD.