毛细管电泳法用于测定大鼠血浆中淫羊藿苷含量的研究

Study on content determination of icariin in rat plasma by capillary electrophoresis

目的采用毛细管电泳法测定大鼠血清中淫羊藿苷的含量。方法用乙酸乙酯萃取,并采用甲醇沉淀的方法处理血浆,以熔融石英毛细管柱(67.4cm×75μm,有效长度51cm)为分离柱,20mmol/L磷酸,100mmol/L SDS(20%乙腈,2%2-丙醇)为缓冲溶液,pH=2.0,检测波长254nm。结果血清中淫羊藿苷线性范围0.12~2.7mg/mL,精密度RSD为1.7%,重复性RSD为3.7%,加样回收率RSD为3.29%。结论毛细管电泳法方法简单,结果准确,重复性好,可用于检测血清中淫羊藿苷的含量。

Objective To adopt the capillary electrophoresis method to determine plasma Icariin content.Methods The extraction used ethyl acetate and the methynol depositing was adopted to treat the plasma.The separation column was fused-silica capillary column（67.4 cm ×75μm,effective length 51 cm）.20 mmol/L phosphoric acid and 100 mmol/L SDS（20%acetonitrile and 2% propanol ethyl carbinol served as the buffer solution,pH2.0,the detection wavelength was 254 nm.Results The linear range of icariin in serum was 0.12-0.27 mg/mL.RSD of precision was 1.7%.RSD of reproducibility was 3.7%.RSD of recovery rate was 3.29%.Conclusion The method is simple and accurate with good reproducibility,and can be used for the content determination of serum icariin.