HRD1对人乳腺癌细胞增殖迁移的影响及其机制初步研究

Effects and mechanism of HRD1 on proliferation and migration of breast cancer cells

目的:研究羟甲基戊二酰辅酶A还原酶降解蛋白1(HMG-Co A reductase degradation protein 1,HRD1)对乳腺癌细胞增殖和迁移能力的影响,初步探讨其潜在机制。方法:通过Western blot和免疫组织化学实验检测HRD1和乳酸脱氢酶B(lactate dehydrogenase B,LDHB)在乳腺癌组织和相应癌旁组织中的表达。用腺病毒感染人乳腺癌细胞株MDA-MB-231和MCF-7以过表达HRD1,观察HRD1和LDHB的表达水平,MTT实验和平板克隆实验检测细胞的增殖能力,Transwell实验检测细胞的迁移能力。结果:与癌组织相比,HRD1在配对的癌旁组织中高表达,LDHB则呈低表达(P<0.05);过表达HRD1能够显著降低MDA-MB-231和MCF-7的增殖和迁移能力,差异有统计学意义(P<0.05)。结论:过表达HRD1可以降低乳腺癌细胞的增殖和迁移能力,其机制可能是通过下调LDHB蛋白的表达实现。

Objective：To investigate effect of HMG-Co A reductase degradation protein 1（HRD1）on the proliferation and migration of breast cancer cells. Methods：The expression of HRD1 and lactate dehydrogenase B（LDHB）was detected by Western blot in breast cancer tissue specimens and matched adjacent normal breast tissues;The expression of HRD1 and LDHB in MDA-MB-231 and MCF-7 cells transfected with adenovirus over-expressed HRD1 were detected by Western blot. Cell MTT assay,colony-formation assay,and transwell assay were performed to detect cell proliferation and cell migration rate,respectively. Results：Compared with the control group,the protein expression of HRD1 in matched adjacent normal breast tissues was increased,whereas,the protein expression of LDHB was decreased（P〈0.05）. A decline in cell proliferation and migration were found in MDA-MB-231 and MCF-7 cells transfected with adenovirus over-expressed HRD1（P〈0.05）. Conclusion：Overexpression of HRD1 results in the inhibition of growth and migration in human breast cancer cells. It downregulates the LDHB protein levels of human breast cancer cells.