黄芪甲苷联合依那普利治疗改善小鼠急性心肌梗死后心功能

Astragaloside-Ⅳ combined with enalapril improves the cardiac function of mice with acute myocardial infarction

目的:探讨黄芪甲苷(astragaloside Ⅳ,AS-Ⅳ)联合依那普利治疗急性心肌梗死小鼠对心功能改善及其促血管新生的作用机制。方法:24只8周龄雄性C57BL/6小鼠随机分为4组:假手术(Sham)组、对照(CTL)组、依那普利(Enalapril)组、黄芪甲苷+依那普利(AS-Ⅳ+Enalapril)组,除Sham组外,对CTL组、Enalapril组、AS-Ⅳ+Enalapril组3组小鼠均行冠状动脉左前降支结扎术构建心梗模型。对Enalapril组(Enalapril 7.5 mg/kg)、AS-Ⅳ+Enalapril组(AS+Ⅳ 10 mg/kg+Enalapril 7.5 mg/kg)小鼠分别以对应药物进行治疗。2周后行超声检查测定心功能。以HE染色观察心脏病理改变。以CD31/VEGFR3免疫荧光染色观察各组血管密度及血管新生情况。结果:与CTL组相比,Enalapril组和AS-Ⅳ+Enalapril组小鼠心功能得到明显改善,且AS-Ⅳ+Enalapril组优于Enalapril组;CD31/VEGFR3双荧光染色显示,与CTL组相比,Enalapril组和AS-Ⅳ+Enalapril组小鼠梗死边缘区血管密度增高,新生血管比率增高,且AS-Ⅳ+Enalapril组优于Enalapril组。结论:黄芪甲苷联合依那普利可有效改善急性心肌梗死后小鼠的心功能,并可促进心肌梗死边缘区血管新生。

Objective：To investigate the effect of astragaloside Ⅳ combined with enalapril on cardiac function after acute myocardial infarction（AMI）in mice. Methods：Twenty-four 8-week old male C57 BL/6 mice were randomly divided into 4 groups,including the sham group,the CTL group,the enalapril group and the AS-Ⅳ＋enalapril group. The CTL group,the enalapril group and the AS-Ⅳ＋enalapril group were subjected to left anterior descending coronary artery ligation as AMI model. Mice in the enalapril group（enalapril 7.5 mg/kg）and the AS-Ⅳ＋enalapril group（AS＋Ⅳ 10 mg/kg ＋ enalapril 7.5 mg/kg）were treated with the corresponding medicine,respectively. Echocardiography was applied to measure cardiac function 2 weeks later. Pathological changes of heart were studied by HE staining. CD31/VEGFR3 double immunofluorescent staining were used to observe the density of vessels and angiogenesis. Results：Compared to the CTL group,the cardiac function was significantly improved in the enalapril group and the ASⅣ＋enalapril group,especially the latter one. Compared to the CTL group,there was significantly more angiogenesis to be observed in the peri-infarction region of the enalapril group and the AS-Ⅳ＋enalapril group. This effect was better in the AS-Ⅳ＋enalapril group.Conclusion：Astragaloside-Ⅳ combined with enalapril can effectively improve the cardiac function of mice after myocardial infarction and promote the regeneration of blood vessels in the peri-infarction region.