响应面优化超声波辅助酶解法制备樱桃核抗氧化多肽的研究

Optimization of Ultrasonic-assisted Enzymatic Hydrolysis of Cherry Stone for Preparing Antioxidant Peptides by Response Surface Methodology

以樱桃核为原料,在单因素试验的基础上,以·OH清除率为评价指标,通过响应面法优化超声波辅助酶解法制备樱桃核抗氧化多肽工艺。结果表明,5%的樱桃核蛋白液在超声波功率170 W,30℃下预处理20 min后,在p H=7条件下加入碱性蛋白酶和胃蛋白酶(1∶1)4 500 U/g协同酶解,酶解温度50℃,酶解时间120 min,在此条件下,樱桃核酶解多肽的·OH清除率为51.98%,与预测值相近。

To prepare antioxidant peptides from cherry stone, the ultrasonic-assisted enzymatic hydrolysis was optimized by response surface method based on single factor experiment with ·OH scavenging rate as evaluation index.The results showed that, the optimal hydrolysis process was as follows： 5% of cherry stone protein solution was pretreated at an ultrasonic power of 170 W and 30 ℃ for 20 min, then equal amount of alkali protease and pepsase（4 500 U/g） were added in the solution at p H 7, and the enzymatic hydrolysis was carried out at 50 ℃ for 120 min.Under these conditions, the ·OH scavenging rate was 51.98%, agreeing with the predicted value.