抗凝血酶-Ⅲ实验室生物参考区间的建立

Establishment of antithrombin-Ⅲ laboratory biological reference interval

目的建立红桥区铃铛阁街社区卫生服务中心实验室抗凝血酶-Ⅲ(AT-Ⅲ)检测的生物参考区间。方法选用法国STAGO公司的STA-Revolution全自动血凝仪,用底物发光法检测120例健康体检者血浆AT-Ⅲ水平,建立本实验室的生物参考区间。并取20例正常标本对该生物参考区间进行验证。结果对120例健康体检者AT-Ⅲ的检测结果进行分析,属于正态分布,计算其95%的参考区间为本实验室的参考区间。本实验室AT-Ⅲ生物参考区间为75.04~116.24。其中进行验证的20例结果均落在该区间内,说明该参考区间有效。结论实验室检测结果的正常与否对临床诊疗有重要的辅助作用,而正确适用的实验室生物参考区间是判断检测结果正常与否的重要依据,因此建立正确适用的实验室生物参考区间具有重要意义。

Objective To establish an biological reference interval for the detection of antithrombin-Ⅲ（AT-Ⅲ） in the laboratory of Hongqiao District Lingdangge Street Community Health Service Center.Methods Use the STA-Revolution automated coagulation instrument which is basically based on the substrate light emitting method from a French company STAGO to detect the plasmic levels of AT-Ⅲ of 120 healthy samples,to establish our laboratory＇s biological reference interval and the interval will be testified by another 20 samples.Results The data collected from 120 healthy people is distributed normally and the95% reference interval of the data is exactly our laboratory＇s reference interval.The biological reference interval of AT-Ⅲ in our laboratory is from 75.04 to 116.24.We have all 20 testified and none of them is beyond the interval which proves the reference interval is valid.Conclusion The normality of laboratory test results has important auxiliary effects on clinical diagnosis and treatment.The correct application of laboratory biological reference interval is an important basis to judge whether the test results are normal or not.Therefore,it is very important to establish a correct and applicable laboratory biological reference interval.