维药一枝蒿中紫花牡荆素成分的分离制备、鉴定及含量测定

Isolation and Elucidation of Casticin from Uighur Medicine Artemisia Rupestris and Its Quantitative Analysis

目的研究维药一枝蒿中指标成分紫花牡荆素的单体制备方法,并建立其含量测定方法。方法利用HPD-450型大孔树脂吸附法提取富集紫花牡荆素,配合Sephadex LH-20柱色谱技术与波谱手段,分离纯化并鉴定其化学结构;含量测定色谱条件:采用色谱柱为Agilent HC-C18(250 mm×4.6 mm,5μm);流动相为甲醇-水(70∶30);柱温30℃,检测波长350 nm,流速1.0 m L·min-1。结果紫花牡荆素纯度为99.7%,在9.42~75.36μg·m L-1(r=0.999 6)内呈良好的线性关系,平均回收率为109.2%(RSD=2.12%)。结论大孔吸附树脂纯化可以获得高纯度紫花牡荆素;建立的含量测定方法简便、准确、稳定,可以用于维药一枝蒿的质量控制。

OBJECTIVE To study isolation and elucidation of casticin from Uighur medicine Artemisia rupestris and its quantitative analysis. METHODS Casticin was isolated and enriched by HPD-450 macroporous absorption resin with silica gel column and Sephadex LH-20 column chromatography. Its structure was elucidated by spectral analysis. HPLC was used to determine the content of casticin from Uighur medicine Artemisia rupestris. The Agilent HC-C18（250 mm×4.6 mm, 5 μm） column was used with mathnol-warter（70∶30） as the mobile phase at a flow rate of 1.0 m L·min^-1. The detection wavelength was set at 350 nm. The column temperature was 30 ℃. RESULTS The casticin content in purified product was up to 99.7%. Casticin was linear over the ranges of 9.42-75.36 μg·mL^-1（r=0.999 6）, and the average recovery rate was 109.2%, RSD was 2.12%. CONCLUSION Highly purified casticin may be obtained effectivly by macroporous absorption resin method. The HPLC method for determination the content of casticin is simple, accurate and steady. It can be used for the quality control of Uighur medicine Artemisia rupestris.