葡萄籽原花青素对酒精性肝损伤小鼠的保护作用

Protective Effects of Grape Seed Procyanidin on Alcoholic Liver Injury in Mice

目的考察葡萄籽原花青素(grape seed procyanidin,GSP)对酒精性肝损伤小鼠的保护作用及其抗炎机制。方法 ICR小鼠60只,♂,随机分成正常对照组、模型组(56%乙醇)、水飞蓟素组(56%乙醇+90 mg·kg-1·d-1水飞蓟素)及GSP高、中、低剂量组(56%乙醇+400,200,100 mg·kg-1·d-1 GSP)。8周后用分光光度法检测血清中ALT、AST水平,肝MDA含量和SOD、GSH-Px活力;酶联免疫法检测肝NF-κB、TNF-α、IL-1β的含量;HE染色检测肝脏病理改变。结果与模型组比较,GSP可以降低酒精性肝损伤小鼠血清中ALT、AST含量及肝组织中MDA、NF-κB、TNF-α、IL-1β含量(P<0.05),提高SOD、GSH-PX活力(P<0.05),减轻肝脏的病理损伤程度(P<0.05)。结论 GSP对酒精性肝损伤小鼠具有保护作用,其机制可能是通过抗氧化和降炎症反应来发挥作用。

OBJECTIVE To study the protective effects and anti-inflammation mechanism of grape seed procyanidin（GSP） on alcoholic liver injury in mice. METHODS Sixty male ICR mice were randomly divided into six group： normal group. model group（56% alcohol）, silymarin group（56% alcohol ＋90 mg·kg^-1 silymarin）, high, medium, low GSP dose group（56% alcohol＋400, 200, 100 mg·kg^-1 GSP）. Eight weeks later, serum alanine aminotransferase（ALT） and aspartate aminotransferase（AST） level, malondialdehyde（MDA） contents, the superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px） activity in liver were measured by spectrophotometry. The contents of NF-κB, TNF-α, and IL-1β in liver were analyzed by ELISA. HE observe the pathological change in the hepatic tissue. RESULTS Compared with the model groups, GSP in the treatment group decreased the levels of serum ALT and AST and the contents of MDA, NF-κB, TNF-α, IL-1β（P〈0.05）, but increased the activity of SOD and GSH-Px in the hepatic tissue. CONCLUSION GSP can reduce the damage of liver tissue in mice with alcoholic liver injury, by reducing oxidative stress and inflammatory reaction.