大鼠胆汁性肝硬化模型的血流动力学特点

Hemodynamic characteristics of biliary cirrhosis in rats

目的从形态学和血流动力学的角度观察大鼠胆汁性肝硬化模型的特点.方法采用胆总管结扎(common bile duct ligation,CBDL)的方法 ,制作胆汁性肝硬化模型.计算模型的肝、脾系数;Masson染色,观察纤维组织再生的情况;门静脉和股动脉插管测定大鼠的门静脉压力(portal pressure,PP)和平均动脉压力(mean arterial pressure,MAP).结果 CBDL 4 wk以后,肝脏体积增大,颜色呈黄绿色,部分肝脏表面有均匀的结节.Masson染色显示,模型大鼠肝组织大量纤维组织增生,形成假小叶.血流动力学结果显示,模型大鼠PP显著升高,MAP显著下降,PP与MAP呈现显著的正相关(R2=0.9096).严重腹水大鼠PP显著低于轻度腹水大鼠.结论大鼠CBDL 4 wk以后,肝硬化形成.门静脉压力受大鼠平均动脉压力和腹水程度的影响.

AIM To elucidate the hemodynamic characteristics of biliary cirrhosis in common bile duct ligated rats. METHODS Biliary cirrhosis was induced in rats by common bile duct ligation（CBDL）. Rats were divided into a sham group and a CBDL group. At the end of 4 weeks, portal pressure（PP） and mean arterial pressure（MAP） were measured directly via catheters. Liver or spleen coefficient was the ratio of liver weight or spleen weight to body weight. Proliferation of fibrous tissue was evaluated by Masson staining. RESULTS At the end of 4 wk, the liver of CBDL rats was yellow in color and enlarged. The surface of some livers was scattered with numerous nodules. Masson staining indicated extensive proliferation of fibrous tissue and pseudolobule. PP of CBDL rats was increased and MAP was decreased compared with sham rats. There was a significant positive correlation between PP and MAP（R^2 = 0.9096）. PP of rats with severe ascites was lower than that of rats with mild or no ascites.CONCLUSION After 4 wk of CBDL, liver cirrhosis and portal hypertension develop. PP is determined by the degree of ascites and MAP.