冻融循环及物理匀浆制备高质量脂肪脱细胞基质

Prepare decellularized adipose tissue by freeze-thaw cycle and mechanical homogenization

目的采用冻融循环及物理匀浆制备脂肪脱细胞基质（Decellularized adipose tissue,DAT）以提高其自发诱导成脂能力。方法利用匀浆法在短时间内对脂肪组织进行脱油,再经后续脱细胞处理后制备高质量DAT（DAT-1）,传统方式制备的DAT（DAT-2）作为对照。检测基质相关蛋白CollagenⅣ和Laminin的含量,并植入小鼠体内验证脂肪再生效果。结果 DAT-1实现了有效的脱细胞处理,且DAT-1较DAT-2有更多基质关键性蛋白CollagenⅣ,Laminin的保留,小鼠体内移植结果进一步表明DAT-1比DAT-2具有更好脂肪再生能力。结论物理匀浆是一种快速高效地去油方式,避免化学试剂对细胞外基质的破坏,提高DAT支架上关键性蛋白的保留,促进了DAT体内脂肪再生。

Objective Decellularized adipose tissue（DAT） was prepared by physical homogenization to improve its ability of spontaneous lipid induction. Methods In this study, we used homogenization method to remove lipid from adipose tissue, a novel DAT（DAT-1） was fabricated after subsequent decellularization process. Ordinary DAT served as control. Results The results revealed that DAT-1 achieved the same decellularization efficiency, but exhibited better retention of ECM components（laminin and collagen Ⅳ） and in vivo recellularization, compared to those with ordinary DAT. Conclusions Mechanical homogenization is a rapid and efficient method to remove lipid from adipose tissue, which could avoid the use of harsh chemical agent and lead to the better retention of ECM components, promoting the in vivo adipose tissue regeneration of DAT.