傣药对叶豆叶中大黄酸的含量测定

RP-HPLC determination of parietic acid in Cassia alata folium

目的:建立傣药对叶豆叶中大黄酸含量的测定方法。方法:采用RP-HPLC法,色谱柱为:Waters Symmetry Shield RP18(150 mm×4.6 mm,5.0μm)。流动相:以乙腈为流动相A,0.1%磷酸溶液为流动相B,流速1.0m L/min,检测波长为430nm,柱温为30℃。按上述色谱条件测定峰面积,以峰面积积分值对进样量进行线性回归。结果:大黄酸溶液浓度在1.35~54μg范围内与峰面积呈良好的线性关系,r=0.9998;平均加样回收率为107.04%,RSD值0.46%(n=6);样品溶液在24 h内稳定,日内精度良好,RSD=1.73%。结论:该方法经方法学验证,可用于傣药对叶豆叶的质量评价。

Objective： To establish determine the content of Dai medicine to be parietic acid. Method ： Methods of the RP-HPLC. Chromatographic column： Waters Symmetry Shield RP18（ 150 mm × 4. 6 mm,5. 0 μm）,186000109. The mobile phase： eluted wifh acetonitrile as mobile phase A. And 0. 1% phosphoricacid solution as the mobile phase B.. The flow rate was 1. 0 m L/min,and the detection wavelength was set at 430 nm,column temperature for 30 degrees. According to the above chromatographic conditions,the peak area was determined,and the linear regression of the sample size was carried out with the peak area integral value. Result： There was a good linear relationship between 1. 35 and 54μg in the injection volume of parietic acid（ r = 0. 9998）. the average recovery rate was 107. 04%,RSD = 0. 46%（ n = 6）. the sample solution is stable within 24 hours,the intra day precision was good,RSD = 1. 73%. Conclusion： This method is stable and accurate,and can be used for quality control of Dai medicinal Cassia alata folium.