竹叶提取工艺的综合性评价

Synthetical Evaluation for Extraction Technology of Bamboo Leaves

建立竹叶的指纹图谱及其多指标成分的含量测定方法，采用主成分分析（PCA）和聚类分析对6种竹叶提取物的特征图谱进行综合性评价。方法：采用DiamonsilPlusC。。色谱柱（4．6mm×250mm，5μm），流动相0．025％甲酸水溶液一乙腈梯度洗脱，流速1mL·min-，柱温30℃，检测波长350nm，进样量10μL。采用“中药色谱指纹图谱相似度评价系统”（2012版）和SPSS22．0软件对6种竹叶提取物样品的监测结果进行分析。结果：6种竹叶提取物样品中4种成分（绿原酸、异荭草苷、荭草苷、异牡荆素）的质量浓度分别为0．08-0．30，0．24-1．64，0．26-0．79，0．16—0．79g·L-。HPLC指纹图谱共标定了11个共有峰，样品sl-s6的指纹图谱相似度均〉0．89。聚类分析将6种竹叶提取工艺聚为3类；PCA提取出了2个主成分，其累计贡献率达98．9％。结论：6种竹叶提取物中绿原酸、异荭草苷、荭草苷和异牡荆素的含量差异性较大。聚类分析和PCA相互补充、互为佐证，二者与竹叶提取工艺指纹图谱的相似度评价相结合可用于竹叶提取工艺的优化，可为竹叶的工业开发提供参考。

Objective： To establish the methods of HPLC fingerprint and quantitative determination of multi-components of bamboo leaves, the synthetical evaluation for six extraction technologies of bamboo leaves were developed by principal component analysis and hierarchical cluster analysis. Method： The optimal conditions of separation and detection were achieved on Diamonsil Plus C1s column （4.6 mm x 250 mm, 5 pμm） with linear gradient elution of acetonitrile-0. 025% formic acid water solution at a flow rate of 1 mL -min-1 The detection wavelength was 350 nm, the column temperature was 30 ℃. Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine （2012 edition） and SPSS 22.0 were used to analyze the data. Result： The content ranges of 4 components （chlorogenic acid, isoorientin, orientin, isovitexin） in six bamboo leaves extract were 0.08-0.30, 0.24-1.64, 0.26-0.79, 0. 16-0.79 g -L-1 , respectively, A total of 11 common peaks were identified in the HPLC fingerprint and the similarities of the six extract were all 〉 0.89. Six extraction technologies of bamboo leaves were divided into 3 categories according to hierarchical cluster analysis. Two principal components were extracted, whose cumulative contribution rate was 98.9%. Conclusion： The contents of chlorogenie acid, isoorientin, orientin and isovitexin in six bamboo leaves extract have a great difference.Hierarchical cluster analysis and principal component analysis complement and support each other, combining with the similarity evaluation, which can be applied to the optimization of extraction technology of bamboo leaves. Meanwhile, the research can provide reference for industrial development of bamboo leaves.