PEG-6000引发对人工诱导老化的远志种子发芽活力影响

Effect of PEG-6000 Priming on Germination Vigor of Artificially Induced Aging Seeds of Polygala tenuifolia

目的：分析不同浓度聚乙二醇6000（PEG-6000）溶液对人工诱导老化远志种子4种发芽指标的影响（发芽率、发芽势、发芽指数和活力指数），阐明远志种子在贮存过程中的衰老规律及PEG引发处理对远志老化种子的影响。方法：以远志种子为试验材料，在高温（45±1）℃，高湿（100％相对湿度）和密闭条件下，人工模拟老化种子条件，对远志种子进行不同程度的老化处理，随后用不同浓度PEG-6000溶液（10％，20％，30％PEG-6000）对已老化的种子进行引发处理。结果：远志老化种子的发芽率、发芽势、发芽指数和活力指数随处理时间的延长呈逐渐降低趋势，老化60h以上的远志种子不再发芽；20％PEG-6000溶液对远志老化种子发芽指标具有促进作用，接近正常种子的发芽指标；10％，30％PEG-6000溶液对远志老化种子发芽指标也有一定的引发效果，但引发效果较差。20％PEG-6000溶液引发能促进老化36h远志幼苗株高、根长、地上部分生物量和地下部分生物量的增加。结论：该研究表明不同浓度PEG一6000溶液处理对远志老化种子有一定修复作用，可以提高其种子活力，但适宜浓度因种子老化时间不同而存在差异，可为种子引发技术在远志种子贮藏上的应用提供基础数据。

Objective： To analyze the effects of different concentrations of polyethylene glycol 6000 （PEG-6000） solution on 4 germination indexes （germination rate, germination potential, germination index and vitality index） of artificially induced aging seeds of Polygala tenuifolia, and clarify the aging rule of P. tenuifolia seeds during storage and the effects of PEG initiation treatment on the aging seeds. Method： Artificial aging of P. tenuifolia seeds was simulated under the air tight conditions at high temperature （45± 1 ） ℃ , high humidity （100% relative humidity）, and then the aging seeds were treated in 10% , 20% and 30% PEG-6000. Result： With the extension of aging treatment time, the above 4 germination indexes of P. tenuifolia seeds showed a gradually decreasing trend, and the seeds with more than 60 h aging time were not germinated; 20% PEG 6000 solution had a promoting effect on the germination indexes of the aging P. tenuifolia seeds, close to the normal seed germination indexes. 10% PEG and 30% PEG also had certain priming effect, but the priming effect was not very good. 20% PEG-6000 solution promoted the plant height, root length, above-ground biomass biomass, and below-ground biomass growth of 36 h P. tenuifolia seeds. Conclusion： This study showed that the PEG-6000solution with different concentrations had certain repair effects on the aging P. tenuifolia seeds, and improved the seeds vigor, but the suitable concentration was different because of the different aging time of the seeds, providing basic data for the application of seed priming technology to the storage of P. tenuifolia seeds.