补肾抑瘤汤含药血清对PC-3细胞Notch1/Jagged1信号通路的影响

Effect of Bushen Yiliu Decoction Medicated Serum on Notch1/Jagged1 Signaling Pathway in PC-3 Cells

目的：通过体外细胞实验研究补肾抑瘤汤对前列腺癌细胞增殖的干预作用及其机制。方法：首先按照血清药理学方法，给予SD大鼠连续灌胃用药7d，取血制备空白血清和补肾抑瘤汤含药血清；然后以人前列腺癌细胞PC-3为模型研究补肾抑瘤汤含药血清对前列腺癌细胞增殖及Notchl／Jaggedl信号通路的影响，噻唑蓝（MTT）比色法检测补肾抑瘤汤含药血清（2．5％，5％，10％，15％）处理PC-3细胞后不同时间点（12，24，36h）的细胞增殖情况，分别应用逆转录一聚合酶链式反应（RT-PCR）及蛋白免疫印迹法（Westernblot）分析补肾抑瘤汤含药血清对PC-3细胞Notchl，JaggedlmRNA和蛋白表达的影响。结果：与空白组比较，经补肾抑瘤汤含药血清处理12，24，36h后，PC-3细胞增殖受到了不同程度的抑制，其中以36h的抑制作用最为显著（P〈0．01）；补肾抑瘤汤含药血清对Pc-3细胞Notchl，JaggedlmRNA和蛋白表达均有抑制作用，尤其是补肾抑瘤汤10％，15％含药血清组抑制效果明显（P〈0．05，P〈0．01）。结论：补肾抑瘤汤含药血清能明显抑制PC．3细胞增殖，作用可能与调控N0tchl／Jaggedl表达有一定相关性。

Objective： To study the effect and mechanism of Bushen Yiliu decoction （BSD） on proliferation of human prostate cancer cells through in vitro experiments. Method： Firstly, according to the serum pharmacology method, Sprague Dawley rats were treated with the drugs by intragastric administration for 7 days continuously to prepare the blank control serum and BSD medicated serum; then, with human prostate cancer cells PC-3 as the models, we observed the effect of BSD on proliferation of human prostate cancer cells and Notchl/ Jaggedl signaling pathway. Methye thiazolye telrazlium （MTT） assay was used to detect the proliferation of PC-3 cells at different time points （ 12, 24, 36 h） after treatment with different concentrations of BSD containing serum （2.5%, 5%, 10%, 15%）; reverse transcription-polymerase chain reaction （RT-PCR） and Western blot methods were used to detect the effects of medicated serum on gene and protein expression levels of Notchl andJagged 1. Result ： As compared with the blank control group, the proliferation of PC-3 cells was inhibited to different degrees after being treated with BSD medicated serum at 12, 24, 36 h, and the effect was most obvious at 36 h （P 〈 O. 01 ）; Furthermore, BSD medicated serum inhibited mRNA and protein expression of Notchlor Jaggedl, especially 10% and 15% medicated serum groups （P 〈 0.05, P 〈 0.01 ）. Conclusion： BSD medicated serum can significantly inhibit the proliferation of PC-3 cells, and the effect may be related to the regulation of Notchl/ Jaggedl expression.