夏天无对坐骨神经损伤小鼠损伤区微环境的影响

Effects of Corydalis decumbens on the microenvironment of area with sciatic nerve injury in mice

目的研究夏天无Corydalis decumbens(Thunb.)Pers.对坐骨神经损伤小鼠损伤区微环境的影响。方法 30只小鼠随机均分为模型组、夏天无组、假手术组,Masson染色检测损伤区胶原纤维、炎性细胞、神经纤维分布,免疫荧光染色检测层黏连蛋白(LN)、施万细胞标记物S-100、小G蛋白Rac1表达及轴突再生情况,HE染色检测神经功能恢复情况。结果与模型组比较,夏天无组胶原纤维、炎性细胞减少,神经纤维有序,LN、Rac1、S-100表达,再生轴突数量,腓肠肌纤维横截面积和腓肠肌质量显著增加(P<0.05)。结论夏天无可通过减少胶原纤维生成和炎症细胞浸润,增加LN、Rac1、S-100表达,改善坐骨神经损伤小鼠损伤区微环境来促进轴突再生和神经功能恢复。

AIM To study the effects of Corydalis decumbens（ Thunb.） Pers. on the microenvironment of area with sciatic nerve injury in mice. METHODS Thirty mice randomly and equally divided into model group,C.decumbens group and sham operation group and had the distributions of collagen fibers,inflammatory cells and nerve fibers in injured area detected by Masson staining,the expressions of laminin（ LN）,Schwann cell marker S-100,small G protein Rac1 and condition of axon regeneration detected by immunofluorescence staining,and the condition of nerve function recovery detected by HE staining. RESULTS Decreased collagen fibers and inflammatory cells,more ordered nerve fibers,more significantly increased expressions of LN,Rac1,S-100,the number of regenerating axons,gastrocnemius muscle fibers in cross section area,and weight of gastrocnemius muscle（ P〈0. 05） were observed in the C. decumbens group when in comparison to the model group. CONCLUSION C. decumbens can improve the microenvironment of area with sciatic nerve injury in mice by decreasing collagen fiber generation and inflammatory cell infiltration,increasing LN,Rac1,S-100 expressions,and promoting axon regeneration and nerve function recovery.