HPLC法同时测定杜仲-当归药对中5种成分

Simultaneous determination of five constituents in drug pair of Eucommia ulmoides-Angelica sinensis by HPLC

目的建立HPLC法同时测定补肾活血药对杜仲-当归中绿原酸、松脂醇二葡萄糖苷、京尼平苷酸、阿魏酸、藁本内酯的含有量。方法药对50%乙醇提取物的分析采用Waters Atlantis T3 C18色谱柱(4.6 mm×250 mm,5μm);以乙腈(A)-0.1%磷酸(B)为流动相,梯度洗脱;体积流量1.0 m L/min;检测波长238 nm(京尼平苷酸)、276nm(松脂醇二葡萄糖苷)、322 nm(绿原酸、阿魏酸和藁本内酯);柱温30℃。结果 5种成分在各自范围内均呈良好的线性关系(r≥0.999 5);平均加样回收率98.29%~103.5%,RSD 1.1%~2.0%。结论该方法准确稳定,重复性好,可用于杜仲-当归药对的质量控制。

AIM To establish an HPLC method for the simultaneous content determination of chlorogenic acid,pinoresinol diglucoside,geniposidic acid,ferulic acid and ligustilid in Eucommia ulmoides-Angelica sinensis（ Bushen Huoxue Herb Couple）. METHODS The analysis of 50% ethanol extract of Eucommia ulmoides-Angelica sinensis was performed on a 30 ℃ Waters Atlantis T3 C18 column（ 4. 6 mm × 250 mm,5 μm）,with the mobile phase comprising of acetonitrile（ A） and 0. 1% phosphoric acid（ B） flowing at 1. 0 m L/min in a gradient elution manner,and the detection wavelength was set at 238 nm（ geniposidic acid）,276 nm（ pinoresinol diglucoside）,322 nm（ chlorogenic acid,ferulic and ligustilid）. RESULTS Five constituents showed good linear relationships within their own ranges（ r ≥ 0. 999 5）,whose average recoveries were 98. 29%-103. 5% with the RSDs of1. 1%-2. 0%. CONCLUSION This accurate,stable and reproducible method can be used for the quality control of Eucommia ulmoides-Angelica sinensis couplet medicines.