VEGF-C拮抗剂对大鼠角膜新生血管的抑制及对移植物成功的影响

Inhibition of VEGF-C antagonist on corneal neovascularization in rats and its effect on graft success

目的研究血管内皮生长因子-C(VEGF-C)拮抗剂对大鼠角膜新生血管的抑制效果以及对角膜移植物成功的影响。方法 48只Wistar大鼠,随机分为低剂量组(给予贝伐单抗5 mg/kg)、中剂量组(给予贝伐单抗10 mg/kg)、高剂量组(给予贝伐单抗15 mg/kg)和对照组(于尾静脉注射等体积生理盐水),每组12只。比较四组大鼠角膜新生血管面积、角膜新生血管最长长度、VEGF-C平均光密度值及角膜移植物成功率。结果低剂量组、中剂量组、高剂量组角膜新生血管最长长度、新生血管面积均低于对照组,且高剂量组新生血管面积为(8.63±0.74)mm2,显著低于低剂量组的(12.15±1.37)mm2,差异具有统计学意义(P<0.05)。低剂量组、中剂量组、高剂量组VEGF-C平均光密度值均显著低于对照组,且高剂量组的VEGF-C平均光密度值显著低于低剂量组,高剂量组、中剂量组移植成功率显著高于对照组,差异具有统计学意义(P<0.05)。结论 VEGF-C拮抗剂对大鼠角膜新生血管的抑制效果显著,可有效降延缓角膜新生血管形成,低降角膜移植排斥反应,提高移植成功率。

Objective To research inhibitory effect of vascular endothelial growth factor-C （VEGF-C） antagonist on corneal neovascularization in rats and its effect on corneal graft success. Methods A total of 48 Wistar rats were randomly divided into low dose group （given Bevacizumab 5 mg/kg）, medium dose group （given Bevacizumab 10 mg/kg）, high dose group （given Bevacizumab 15 mg/kg） and control group （injection of equal volume of physiological saline by caudal vein）, 12 rats in each group. The area of corneal neovascularization, the longest length of corneal neovascularization, the mean optical density of VEGF-C and the success rate of corneal graft among the rats of four groups were compared. Results The longest length of corneal neovascularization and the area of corneal neovascularization in the low dose group, medium dose group and high dose group were significantly lower than that in the control group, and the area of corneal neovascularization in the high dose group was （8.63±0.74） mm2, which was significantly lower than （12.15±1.37） mm2 in the low dose group, the differences were statistically significant （P〈0.05）. The mean optical density of VEGF-C in the low dose group, medium dose group and high dose group were significantly lower than that in the control group, and the mean optical density of VEGF-C in the high dose group was significantly lower than that of the low dose group, the success rate of corneal graft in the high dose group and medium group were significantly higher than that in the control group, the differences were statistically significant （P〈0.05）. Conclusion The inhibitory effect of VEGF-C antagonist on corneal neovascularization is significant. It can effectively delay the formation of corneal neovascularization, lower the rejection reactions of corneal transplantation and improve the success rate of transplantation.