摘要
采用实时荧光定量PCR法分析了牛磺鹅去氧胆酸(taurochenodeoxycholic acid,TCDCA)对佐剂性关节炎模型(adjuvant arthritis,AA)大鼠成纤维滑膜细胞(fibroblast-like synoviocytes,FLS)中由糖皮质激素受体(glucocorticoid receptor,GR)介导的热休克蛋白90基因(heat shock protein 90 gene,HSP90)mRNA表达的影响。结果表明,TCDCA可以极显著性(P<0.01)下调FLS细胞内HSP 90 mRNA的表达,同时加入GR的特异性抑制剂RU486后可抑制TCDCA对HSP 90 mRNA的作用。由此证明了TCDCA是通过GR受体发挥抗炎和免疫调节作用的。
The effect of taurochenodeoxycholic acid （TCDCA） on HSP90 mRNA expression mediated by glucocorticoid receptor （GR） in fibroblast-like synoviocytes （FLS） in rats with adjuvant arthritis （AA） was assessed by real-time quantitative PCR assay. The results showed that TCDCA extremely significantly down-regulated the HSP90 mRNA expression in FLS cells （P〈 0.01 ）. In addition, the effect of TCDCA on HSP90 mRNA expression could be inhibited by RU486, a specific inhibitor against GR. It was indicated that TCDCA exerted anti-inflammatory and immunoregulative action via GR.
作者
宝力格
周蕾
郝大成
朱相成
罗宏亮
李培锋
Baoligel,;ZHOU Lei;HAO Da-cheng;ZHU Xiang-cheng;LUO Hong-liang;LI Pei-feng(College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease,Ministry of Agriculture,Hohhot 010018,China;The Spirit Jinyu Biological Pharmaceutical Co.,Ltd,Hohhot 010030,China)
出处
《畜牧与饲料科学》
2018年第8期1-3,共3页
Animal Husbandry and Feed Science
基金
国家自然科学基金资助项目(31160518)
