中期因子蛋白mRNA在非小细胞肺癌组织中的表达及其与肿瘤预后和淋巴结转移的关系

Expression of midkine mRNA in non-small cell lung cancer and its relationship with prognosis and lymph node metastasis

目的:探讨中期因子蛋白(midkine,MK)m RNA在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中的表达及其与肿瘤预后和淋巴结转移的关系。方法:2013年2月到2016年6月选择我院收治的72例NSCLC患者的手术切除病灶标本作为观察组,同期选择远离肿瘤组织≥3 cm的癌旁组织72例作为对照组,所有标本提取组织RNA后进行MK m RNA逆转录PCR分析,记录患者的临床特征、淋巴结转移,并随访预后情况。结果:观察组MK m RNA相对灰度比值为8.12±2.13,明显高于对照组的1.29±0.82(t=12.493,P<0.05)。在观察组中,分化程度越低、肿瘤越大、临床分期越高的患者,MK m RNA相对灰度比值越高(P<0.05)。观察组所有患者随访1年,死亡12例,存活60例;死亡患者与淋巴结转移患者的MK m RNA相对灰度比值明显高于存活患者与无淋巴结转移患者(P<0.05)。Spearman相关分析显示观察组的MK m RNA表达与患者预后死亡、淋巴结转移有明显的正相关性(r=0.563,0.492,P<0.05)。结论:MK m RNA在NSCLC组织中呈现高表达状况,与患者的预后及淋巴结转移有明显相关性,有很好的检测诊断价值。

Objective： To investigate the expression of midkine（ MK） m RNA in non-small cell lung cancer（ NSCLC） tissue and its relationship with tumor prognosis and lymph node metastasis. Methods： From February 2013 to June 2016,72 cases of surgically resected specimens of NSCLC patients in our hospital were selected as the observation group,and the other 72 cases（ ≥3 cm away from tumor tissue） were selected at the same period as the control group. All specimens were given MK m RNA extraction and RT-PCR analysis,and the clinical features,lymph node metastasis and prognosis in the patients were recorded. Results： The relative gray ratio of MK m RNA in the observation group was 8. 12 ± 2. 13,which was significantly higher than that of the control group（ 1. 29 ± 0. 82）（ t = 12. 493,P〈0. 05）. In the observation group,when patients had the lower differentiation,the larger tumor and the higher clinical stage,there were higher the relative gray ratio of MK m RNA（ P〈0. 05）. All the patients in the observation group were followed up for 1 year. 12 cases were died,and 60 cases were survived. The relative gray ratio of MK m RNA in the patients with death and lymph node metastasis were significantly higher than that in the patients with survival and without lymph node metastasis（ P〈0. 05）. Spearman correlation analysis showed that the expression of MK m RNA in the observation group had positive correlation with tumor prognosis,death and lymph node metastasis（ r = 0. 563,0. 492,P〈0. 05）. Conclusion： The expression of MK m RNA in NSCLC tissues is high,and is correlated with the prognosis of patients and lymph node metastasis. It has a good value in diagnosis.