草莓不同生长时期土壤微生物群落结构变化

Variation of Soil Microbial Community Structure at Different Growth Stages of Strawberry

为制定草莓的适时施肥计划提供理论依据,采用Illumina高通量测序平台对草莓不同生长时期土壤微生物的群落进行16SrRNA基因(V3-V5区)高通量测序,检测4个草莓不同生长时期的土壤微生物群落结构和丰度。结果表明:测序共获得有效序列数364 968条,OTU数38 124个,归属为10门(Proteobacteria, Acidobacteria, Chloroflexi, Actinobacteria, Saccharibacteria, Thaumarchaeota, Bacteroidetes,WD272,Gemmatimonadetes,Nitrospirae)和其他未分类的单元;结果期土壤微生物群落的丰度值最大,特有的微生物种类也最多;优势微生物菌群为变形菌门(Proteobacteria)和酸杆菌门(Acidobacteria),生长期和结果期的优势属均为(Candidatus Solibacter),开花期和盛果期的优势属为副球菌属(Rhizomicrobium)。

The soil microbial community structure and abundance were determined by 16SrRNA gene （V3-V5 region） based on an Illumina high-throughput sequencing platform at four growth stages of strawberry to provide the theoretical basis for formulating the timely fertilization plan in strawberry production. Result： 364 968 effective sequences and 38 124 OTU obtained by the sequencing method belong to （ Proteobacteria, Aeidobacteria： ：Chloroflexi： Actinobacteria, Saccharibacteria, Thaumarchaeota, Bacteroidetes： WD272, Gemmatimonadetes： Nitrospirae） and other unclassified units. The abundance value of soil microbial community and specific microbial species both are the highest at fruit-bearing stage of strawberry. The dominant microbial communities are Proteobacteria and Acidobacteria. The dominant genus of soll microbial community is Can didatus Solibacter at growth stage of strawberry but the dominant genus of soil microbial community is Rhizomicrobium at flowering and ful1 bearing stage of strawberry.