化学发光法检测育龄妇女单纯疱疹病毒结果分析

Detection of Herpes Simplex Virus in Women of Child-bearing Age by Chemiluminescence

目的使用化学发光免疫分析(CLIA)技术对孕前及孕早期育龄妇女进行单纯疱疹病毒(herpes simplex virus,HSV)检测分析,了解本地区孕前及孕早期妇女单纯疱疹病毒的感染状况,为制定孕前及孕期治疗保健措施提供依据。方法对2015年1月—2018年4月来我院进行孕前检查和孕早期建卡的1 204例育龄妇女进行单纯疱疹病毒1+2型(HSV-1+2)特异性抗体Ig M和Ig G检测,并对检测结果进行统计分析。结果 1 204例育龄妇女中HSV-1+2-Ig M总阳性率为19.10%,HSV-1+2-Ig G总阳性率为73.92%。孕前组HSV-1+2型Ig M和Ig G阳性率低于孕早期组,但二者比较差异无统计学意义(P>0.05)。结论应用CLIA法联合检测HSV特异性抗体Ig M和Ig G,更有利于帮助查明是最近感染、再次感染,还是既往感染。孕前及孕早期育龄妇女应将单纯疱疹病毒作为常规检测项目,对阳性患者早做预防、治疗、处理,有效提高人口素质、减少各种缺陷性疾病发生。

Objective Chemiluminescence immunoassay was used to detect herpes simplex virus in women of childbearing age before and during early pregnancy,objective to understand the infection status of herpes simplex virus in prepregnant and early pregnant women in the region,and to provide evidence for making health care measures before and during pregnancy.Methods 1 204 women of childbearing age from January 2015 to April 2018 in our hospital were tested for the specific antibody Ig M and Ig G of herpes simplex virus 1＋2（HSV-1＋2）,and the results were statistically analyzed.Results The total positive rate of HSV-1＋2-Ig M in 1 204 women of childbearing age was 19.10%,and the total positive rate of HSV-1＋2-Ig G was 73.92%.The positive rate of HSV-1＋2 Ig M and Ig G in pre pregnancy group was lower than that in early pregnancy group,but there was no significant difference between the two groups（P 〉0.05）.Conclusion The application of CLIA to detect HSV specific antibodies Ig M and Ig G is more conducive to identifying recent infection,reinfection or previous infection.Pre-pregnancy and early pregnancy women of childbearing age should take herpes simplex virus as a routine test project to prevent,treat and treat the positive patients early,effectively improve the quality of population and reduce the occurrence of all kinds of defective diseases.