脂肪干细胞异位成骨修复兔上颌大面积缺损效果

Effect of the ectopic bone of adipose-derived stem cells in the repair of large area defect of maxillary

目的:探讨脂肪干细胞异位成骨修复上颌骨大面积缺损的临床效果。方法:建立兔上颌缺损模型,取自体脂肪干细胞进行异位成骨修复,活体检查及影像学检查观察修复效果。结果:活检结果显示,实验动物新生骨组织血管化良好,为成熟骨组织结构。重建术后2个月,腹直肌基本上完全萎缩,上皮化良好。丙烯酸固定桥修复成功,修复功能良好率为95.0%。术后6周,常规下颌CT扫描,并进行三维重建。实验动物新生骨量明显,缺损边缘及中央均有明显新骨生成,术后8周,缺损已经完全修复。结论:脂肪干细胞异位成骨修复兔上颌骨大面积缺损无明显排斥反应,值得临床广泛推广。

Objective： To investigate the clinical effects of the ectopic bone of adipose-derived stem cells in the repair of large area defect of maxillary. Methods： The maxillary defect model of rabbit was established,and the defect was repaired with ectopic bone of autologous adipose-derived stem cells. The repair effect was observed using biopsy and imaging examination. Results： The biopsy results showed that the new bone tissue vascularization was good and mature bone tissue structure. After 2 months of reconstruction,the rectus abdominis was basically completely atrophic,and the epithelialization was good. Acrylic fixed bridge restoration was successful,the good rate of function recovery was 97. 9%. After 6 weeks of operation,the underjaw was detected using the conventional submandibular CT scan,and the 3 D reconstruction of which was implemented. The new bone was obvious,and the new bone was identified in the center and edge of defect. After 8 weeks of operation,the defect had been completely restored. Conclusions： The ectopic bone of adiposederived stem cells in the repair of large area defect of maxillary has no obvious rejection reaction,which is worthy of clinical application.