复合植物精油对脂多糖诱导的断奶仔猪免疫应激的影响

The Effect of Compound Plant Essential Oil on LPS-induced Immune-stressed Weaned Pigs

试验旨在研究复合植物精油(OCT)对脂多糖(LPS)诱导的断奶仔猪的免疫应激的影响。试验选取28日龄左右的杜×长×大三元杂交仔猪,随机分成3个处理组:对照组(基础日粮,注射生理盐水),LPS组(基础日粮,注射LPS),OCT组(基础日粮+50mg/kg OCT,注射LPS)。每组8个重复,每个重复1头猪。于试验第21天注射LPS(100μg/kg体重)或等体积的生理盐水,注射3h后前腔静脉采血进行白细胞分类计数;6h后屠宰,取胸腺、脾脏,-80℃保存,用于测量胸腺、脾脏免疫相关基因白介素-4(IL-4)、白介素-6(IL-6)、白介素-10(IL-10)、肿瘤坏死因子(TNF-α)、核因子(NF-κB)以及转录相关基因转录激活因子3(STAT3)的基因相对表达量。结果表明:(1)与对照组相比,LPS刺激降低了仔猪血液中白细胞、中性粒细胞、淋巴细胞、单核细胞及嗜酸性粒细胞数量(P<0.05);与LPS组相比,日粮中添加OCT提高了白细胞的数量(P<0.05);(2)与对照组相比,LPS刺激上调了脾脏、胸腺IL-6、STAT3mRNA水平(P<0.05),下调了脾脏IL-4、TNF-α及胸腺IL-4、TNF-α、NF-κB mRNA水平(P<0.05);与LPS组相比,添加OCT下调了脾脏、胸腺IL-6、STAT3mRNA水平(P<0.05);上调了胸腺IL-4、IL-10、TNF-α、NF-κB的mRNA水平(P<0.05)。综合上述结果,LPS诱导发生了仔猪免疫应激,OCT在一定程度具有缓解作用。

The purpose of this research was to study the effect of OCT on the immune stress of weaned piglets induced by LPS.A total of 24 weaned piglets（Duroc×Large white×Landrace）were randomly assigned to three treatments at 28 days：Control group（based diet,injection of saline）,LPS group（basic diet,injection of LPS）and OCT group（basal diet＋50 mg/kg OCT,injection of LPS）.Each treatment included 8 piglets that were subdivided equally to 8 pens,each pen had 1 piglets.On 21 th day of the experiment,piglets in the LPS and OCT groups were injected intraperitoneally with LPS（100μg/（kg·BW））,whereas piglets in the control group were injectedintraperitoneally with the same volume of physiological saline.Blood samples were obtained 3 h after injection for analysis of white blood cell differential counts.6 h after LPS challenged,the pigs were slaughtered and thymus and spleen were taken for measuring the related genes IL-4,IL-6,IL-10,TNF-α,NF-κB,and STAT3 mRNA.The result showed that：（1）Compared with control group,LPS stimulation could lead to a significant reduction in the number of the eosinophils,neutrophils,lymphocytes,monocytes,white blood cells in the blood（P〈0.05）.Compared with LPS group,adding OCT could significantly increase blood levels of white blood cells（P〈0.05）.（2）Compared with control group,after injection of LPS,mRNA levels of IL-6 and STAT3 genes significantly increased in spleen and thymus（P〈0.05）;mRNA levels of IL-4,TNF-αin spleen and IL-4,TNF-α,NF-κB genes mRNA levels in thymus had been significantly reducted（P〈0.05）.Compared with LPS group,adding OCT could significantly reduce IL-6 and STAT3 mRNA levels in both spleen and thymus（P〈0.05）,and significantly increase IL-4,IL-10,TNF-αand NF-κBgenes mRNA levels in thymus（P〈0.05）.In conclusion,LPS could induce immune stress in weaned pigs,whereas OCT could improve its immunity and alleviate inflammation to some extent.