基于Au@Ag/碳纳米管的SERS-荧光双模探针癌细胞成像应用研究

A SERS and Fluorescence Dual Mode Cancer Cell Imaging Probe Based on Au@Ag Nanoparticles Functionalized Carbon Nanotubes

报告了一种基于Au@Ag/碳纳米管(CNTs)的SERS-荧光双模细胞成像探针,其可以产生表面增强拉曼散射(SERS)和荧光信号。在这种探针中,金/银核壳纳米粒子(Au@Ag NPs)作为SERS增强基底首先通过静电作用组装到碳纳米管表面,然后标记4-巯基苯甲酸(4MBA)以产生SERS信号,接着通过氨基与硫碳胺键作用将异硫氰酸荧光素(FITC)和碳纳米管连接以产生荧光信号。使用HeLa细胞作为模型癌细胞,通过荧光和SERS成像结果证实了所提出的探针可以实现细胞成像功能。

We report a dual mode cancer cell imaging probe based on Au@Ag nanoparticles functionalized carbon nanotubes,which can generate both surface enhanced Raman scattering（SERS）and fluorescence signals.In such a probe,gold/silver core-shell nanoparticles（Au@Ag NPs）are adsorbed on the surfaces of MWCNTs through an electrostatic interaction,which serve as the SERS substrate.Then the Au@Ag NPs are labeled with 4-mercaptobenzoic acid（4 MBA）to generate SERS signals.Finally,FITC are conjugated to the carbon nanotubes by the amino and thiocarbonic bonds to yield fluorescence.Using HeLa cells for in vitro experiments,both the fluorescence and SERS imaging results confirm that the proposed probe can be used as an efficient cancer cell imaging probe.