盲肠灌注丙酸对生长猪结肠黏膜基因表达的影响

Effects of Cecal Infusion of Propionate on Gene Expression in Colon Mucosa of Growing Pigs

本试验旨在通过盲肠灌注丙酸,利用转录组测序,探究丙酸对生长猪结肠黏膜基因表达的影响。试验选取16头杜×长×大阉公猪,随机分成对照组和试验组,每组8头。试验期内,通过盲肠瘘管给对照组和试验组试验猪分别灌注等量的生理盐水及用生理盐水配制的丙酸溶液,共灌注28 d。试验期结束后屠宰,采集结肠黏膜进行转录组测序分析。结果显示:灌注丙酸后,在生长猪结肠黏膜中共检测出121个差异表达基因[差异倍数(FC)≥2,P<0.05],其中78个基因上调,43个基因下调。其中,丙酸显著提高了酪酪肽(PYY)、胰岛素样生长因子结合蛋白7(IG FBR7)、果糖二磷酸醛缩酶(ALDOB)、ATP合成酶5I(ATP5I)、烟酰胺腺嘌呤二核苷酸脱氢酶亚基4L(ND4L)、细胞色素P450(CYP39A1)等糖代谢和能量代谢相关基因的表达(P<0.05),显著促进了闭锁蛋白-1(Occludin-1)、生长抑素(SST)、G蛋白偶联受体5A(G PRC5A)等免疫相关基因的表达(P<0.05)。经GO与KEGG pathway分析发现,上调基因显著富集的GO term主要包括糖基化合物代谢、糖类衍生物代谢等,显著富集的通路有氧化磷酸化、糖胺聚糖生物合成等。下调基因显著富集的GO term主要有心腔发育、干细胞群维持等,显著富集的通路包括牛磺酸和亚牛磺酸代谢、叶酸生物合成等。综上所述,盲肠灌注丙酸改变了结肠糖代谢和能量代谢,以及肠道屏障与免疫功能相关基因的表达,表明丙酸对结肠代谢及机体健康有一定的调节作用。

The aim of this experiment was to explore the effects of cecal infusion of propionate on gene expression in colon mucosa of growing pigs via RNA sequencing（ RNA-seq）. Sixteen growing barrows（ Duroc ×Landrace×Large White） were divided into control group（ n = 8） and experimental group（ n = 8） randomly.The pigs from the experimental group were infused with propionate which was formulated with saline through the cecal fistula,while the pigs from the control group were infused with the same amount of saline for 28 days. After the infusion,pigs were slaughtered,and their colon mucosas were collected for RNA-seq analysis.The results showed that there were 121 differentially expressed genes in colon mucosa after infusion of propionate（ FC ≥2,P 〈 0.05）,with 78 up-regulated and 43 down-regulated genes. In which,propionate significantly increased the expression of glucose-and energy-related genes,including peptide YY（ PYY）,insulin-like growth factor binding protein-7（ IG FBP7）,fructose-bisphosphate aldolase（ ALDOB）,ATP synthase 5 I（ATP5 I）,nicotinamide adenine dinucleotide dehydrogenase subunit 4 L（ ND4 L） and cytochrome P450（ CYP39 A1）（P0.05）. In addition,the expression of immune-related genes,such as Occludin-1,somatostatin（ SST） and G protein-coupled receptor（ G PRC5 A） was also increased（ P 〈 0.05）. GO and KEGG pathway analysis showed that the up-regulated DEGs significantly enriched GO term involved in glycosyl compound metabolism,carbohydrate derivative metabolism,etc,and significantly enriched pathways involved in oxidative phosphorylation,glycosaminoglycan biosynthesis,etc; the down-regulated DEGs significantly enriched GO term involved in cardiac chamber development,stem cell population maintenance etc,and significantly enriched pathways involved in taurine and hypotaurine metabolism,folate biosynthesis,etc. Taken together,cecal infusion of propionate alters the expression of glucose-and energy-related genes,and intestinal barrier-and immune-related genes,suggesting a crucial role of propionate in colonic metabolism and host health.