菊花中硒定量分析方法的建立与应用

Establishment and application of quantitative analysis method for selenium in Chrysanthemum morifolium

为运用氢化物原子荧光光谱法测定陕西省市售菊花样品的硒含量。采用单因素试验和正交试验优化得到氢化物原子荧光光谱法的最佳测定条件为:负高压为320 V,灯电流为85 mA,硼氢化钠质量分数为1.0%。系统适应性试验结果显示,该方法的标准曲线方程为Y=116.86x+88.69(r=0.999 8),线性范围为10.00~100.00μg/L,检出限(LOD)、定量限(LOQ)分别为2.60μg/L、7.88μg/L,精密度为1.27%,平均回收率为98.38%。该方法精确、灵敏、简便、安全,分析成本较低。测定发现,陕西省各地市售菊花样品中硒的含量存在较大差异。

A hydride generation atomic fluorescence spectrometric method wasused to detect the content of selenium in Chrysanthemum morifolium samples purchased from Shaanxi province. The optimal conditions for the hydride generation atomic fluorescence spectrometry obtained by single factor experiments and orthogonal test were as follows： negative high voltage of 320 V,lamp current of 85 m A,and NaBH4 mass fraction of 1. 0%. System suitability test results indicated that regression equation of standard curve was Y = 116.86 x＋88. 69,the correlation coefficient（ r） was 0.999 8,and linear range was 10.00-100. 00 μg/L. Limits of detection（ LOD） and limits of quantitation（ LOQ） were 2. 60 μg/L and 7. 88 μg/L,respectively. Precision and average recovery were 1. 27% and 98. 38%,respectively. The method is accurate,sensitive,simple,safe as well as low-cost. A large variation of selenium contents was found among Chrysanthemum morifolium samples from different regions in Shaanxi province.