SFRP2单克隆抗体对肾癌SNU349细胞增殖及迁移的影响

The effects of anti-SFRP2 monoclonal antibody on cell proliferation and migration of renal cell carcinoma SNU349 cells

目的探讨分泌型卷曲相关蛋白2(SFRP2)在肾透明细胞癌与正常肾组织中的表达情况,研究SFRP2单克隆抗体对肾癌SNU349细胞株增殖、迁移的影响。方法采用基因芯片分析及qRT-PCR检测37例肾透明细胞癌组织及10例癌旁正常组织中SFRP2的表达情况;采用细胞增殖试验及划痕试验检测不同浓度的SFRP2单克隆抗体对SFRP2高表达株肾癌SNU349细胞体外增殖、迁移的影响。结果PCR芯片及qRT-PCR结果显示,SFRP2在部分肾透明细胞癌组织中表达明显增高(P<0.05);SFRP2单克隆抗体能有效抑制肾癌SNU349细胞的体外增殖和迁移,其抑制作用呈浓度依赖性(P<0.05)。结论SFRP2在部分肾透明细胞癌组织中显著高表达,其表达程度与肿瘤分期无明显关系。SFRP2单克隆抗体能有效抑制肾癌SNU349细胞体外增殖、迁移,提示SFRP2可能是相应SFRP2高表达型肾癌的治疗靶点之一。

Objective To investigate the expression of SFRP2 in clear cell carcinoma（ccRCC）and to explore the effects of anti SFRP2 monoclonal antibody（SFRP2 mAb）on the proliferation and migration of renal cell carcinoma SNU349 cells.Methods The expression of SFRP2 was detected by significance analysis of microarrays（SAM）from E-GEOD-53757 dataset and qRT-PCR in 37 cases of ccRCC and 10 cases of adjacent normal renal tissue.The proliferation and migration of ccRCC SNU349 cells were detected by MTT assay and the scratch test administration with different concentrations of SFRP2 mAb.Results SFRP2 was highly expressed in some samples of human ccRCC tissues.SFRP2 mAb inhibited of the proliferation and migration of the ccRCC SNU349 cells in a dose-dependent manner（P〈0.05）.Conclusion The expression of SFRP2 was high in some cases of ccRCC and SFRP2 mAb can effectively inhibit the proliferation and migration of SNU349 cells in vitro,which indicates the possibility of SFRP2 to be a promising therapeutic target.